Protection of sheep and cattle against haemoncose after immunization with antigens from the intestinal membrane of Haemonchus contortus.

• Grant number: 10/18678-5
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: March 01, 2011
• End date: February 28, 2015
• Field of knowledge: Biological Sciences - Parasitology - Helminthology of Parasites
• Principal Investigator: Alessandro Francisco Talamini Do Amarante
• Grantee: César Cristiano Bassetto
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

This study will be carried out to assess the protection of a vaccine against haemoncose in sheep and cattle. The vaccine will be made up of antigens from the H. contortus intestinal membrane. Two experiments will be performed: in the first, the vaccine will be given to periparturient ewes and in their lambs. Thirty-three ewes of Bergamacia breed will be distributed in three experimental groups: Group 1 (n=11), control, vaccinated only with QuilA adjuvant; Group 2 (n=11), vaccinated with 5 µg of antigens; Group 3 (n=11), vaccinated with 50 µg of antigens. Four doses of vaccine will be given to the ewes: three doses prior to lambing and one dose four weeks after lambing. The lambs born from ewes in all groups will also be in other three experimental groups: Group 1, control; Group 2, vaccinated with 5 µg of antigens; Group 3, vaccinated with 50 µg of antigens. Lambs will receive five doses of the vaccine, the first three doses with intervals of three weeks each and the last two doses with intervals of six weeks. When the lambs complete 26 weeks of age, euthanasia will be performed in order to collect and quantify the gastrointestinal worms. The second experiment will be carried out with 24 Nelore calves, which will be divided into two groups: one is the control treated as previously and the other is vaccinated seven times with 50 µg of antigens for seven months. As calves complete 55 weeks of age, euthanasia will be performed in order to collect and quantify the gastrointestinal worms. In both experiments faecal, blood and immunological examinations will be performed, data will be submitted to analyses of variance, and means will be compared by Student's t test.

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DA SILVA, BRUNA FERNANDA; BASSETTO, CESAR CRISTIANO; TALAMINI DO AMARANTE, ALESSANDRO FRANCISCO. Immune humoral response of young lambs naturally infested by Oestrus ovis (Diptera: Oestridae). Revista Brasileira de Parasitologia Veterinária, v. 27, n. 3, p. 295-300, JUL-SEP 2018. (10/18678-5)

BASSETTO, C. C.; PICHARILLO, M. E.; NEWLANDS, G. F. J.; SMITH, W. D.; FERNANDES, S.; SIQUEIRA, E. R.; AMARANTE, A. F. T.. Attempts to vaccinate ewes and their lambs against natural infection with Haemonchus contortus in a tropical environment. International Journal for Parasitology, v. 44, n. 14, p. 1049-1054, DEC 2014. (10/18678-5, 11/03806-0)

BASSETTO, C. C.; SILVA, M. R. L.; NEWLANDS, G. F. J.; SMITH, W. D.; RATTI JUNIOR, J.; MARTINS, C. L.; AMARANTE, A. F. T.. Vaccination of grazing calves with antigens from the intestinal membranes of Haemonchus contortus: effects against natural challenge with Haemonchus placei and Haemonchus similis. International Journal for Parasitology, v. 44, n. 10, p. 697-702, SEP 2014. (10/18678-5)

SANTOS, MICHELLE C.; XAVIER, JORGE K.; AMARANTE, MONICA R. V.; BASSETTO, CESAR C.; AMARANTE, ALESSANDRO F. T.. Immune response to Haemonchus contortus and Haemonchus placei in sheep and its role on parasite specificity. Veterinary Parasitology, v. 203, n. 1-2, p. 127-138, JUN 16 2014. (10/18678-5, 11/09779-5, 11/03706-6)

DA SILVA, BRUNA FERNANDA; BASSETTO, CESAR CRISTIANO; TALAMINI DO AMARANTE, ALESSANDRO FRANCISCO. Immune humoral response of young lambs naturally infested by Oestrus ovis (Diptera: Oestridae). REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA, v. 27, n. 3, p. 6-pg., 2018-07-01. (10/18678-5)

BASSETTO, C. C.; AMARANTE, A. F. T.. Vaccination of sheep and cattle against haemonchosis. JOURNAL OF HELMINTHOLOGY, v. 89, n. 5, p. 517-525, SEP 2015. (10/18678-5)

AMARANTE, M. R. V.; BASSETTO, C. C.; NEVES, J. H.; AMARANTE, A. F. T.. Species-specific PCR for the identification of Cooperia curticei (Nematoda: Trichostrongylidae) in sheep. JOURNAL OF HELMINTHOLOGY, v. 88, n. 4, p. 447-452, DEC 2014. (10/18678-5)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

BASSETTO, César Cristiano. Proteção de ovinos e bovinos contra haemonchose após imunização com antígenos oriundos da membrana intestinal de Haemonchus contortus. 2015. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Instituto de Biociências. Botucatu Botucatu.