Gene expression profile of endometrial tissue in female dogs at diestrus phase, females affected by cystic endometrial hyperplasia, mucometra and pyometra

• Grant number: 11/02437-1
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: July 01, 2011
• End date: December 31, 2013
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: Gilson Hélio Toniollo
• Grantee: Fabiana Azevedo Voorwald
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

Pyometra is an acute or chronic suppurative and degenerative inflammation of endometrium, characterized by exudate accumulation in endometrial glands and uterine lumen, as consequence of exacerbate endometrial response of chronic endogenous or exogenous progesterone and estrogen influence, as a result of bacteremia, hepatorenal syndrome and toxemia. The progressive and degenerative process development by cystic endometrial hyperplasia (CEH), due to glandular distension and fibrosis, is proposed as initial lesion of pyometra. Studies demonstrated that the paradigm of progesterone resulting in cystic endometrial hyperplasia, which results in pyometra, may not be always correct; instead, bacteria may be the initial causative agent of pyometra. The development of molecular biology techniques allows detection of thousands of genes simultaneously, in order to identify those involved in the pathogenesis of the studied disease. Microarrays are a powerful method for the global analysis of gene or protein content and expression, opening up new horizons in molecular and physiological systems. In the last few years, both cDNA and oligonucleotide microarray technology have been successfully applied to the study of endometrial gene expression. The endometrium is a uniquely dynamic tissue, with the capacity to undergo dramatic remodeling in response to cyclic variations in steroid hormones and local autocrine and paracrine factors. It aims to analyze and compare the gene expression and identify differentially expressed genes, responsible to endometrial proliferation and hyperplasia of female dogs affected by CEH, females affected by mucometra and pyometra, comparing to the normal endometrial tissue of female dogs at diestrus phase, by microarray technique, and confirm the obtained results to selected genes by reverse transcriptase technique - real-time polymerase chain reaction (RT-PCR) or immunohistochemistry, and investigate the relationship between the differentially expressed genes with the clinical staging and survival. Explain the mechanisms involved to pathophysiology of cystic endometrial hyperplasia, mucometra and pyometra, to clarify the expression gene profile and the involved genes function, will elucidate the molecular environment of the endometrial lesions and new molecular markers can be identified, allowing early diagnosis and more effective and less invasive treatments. (AU)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

VOORWALD, Fabiana Azevedo. Aspectos clínicos, histopatológicos e expressão gênica do endométrio de cadelas acometidas por hiperplasia endometrial cística, mucometra e piometra. 2014. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal Jaboticabal.