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Uterine hemodynamic, inflammatory and proliferative changes in cystic endometrial hyperplasia-piometra bitches

Grant number: 12/22958-9
Support Opportunities:Regular Research Grants
Duration: March 01, 2013 - February 28, 2015
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Reproduction
Principal Investigator:Camila Infantosi Vannucchi
Grantee:Camila Infantosi Vannucchi
Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil


The presumptive diagnosis of Cystic Endometrial Hyperplasia (CEH)-Pyometra Complex is generally determined through two-dimensional ultrasound examination. However, differential diagnosis between CEH-mucometra and pyometra is achieved by the combined analysis of ultrasonographic findings, clinical signs, as well as uterine macroscopic and histological evaluation. In order to establish an accurate, precocious and noninvasive method of diagnosis, the aim of this study was to characterize uterine blood flow in bitches with distinct uterine pathological conditions. For this purpose, 25 bitches will be allocated into 3 groups, according to clinical signs, ultrasound analysis and uterine histological examination: Diestrus Group (n = 5), CEH-mucometra Group (n = 10); and Pyometra Group (n = 10). Scanning of the uterine horns by colour Doppler will be performed for quantitative assessment of the overall uterine vascularization. An arbitrary score from 1 to 4 will be adopted, being score 1 the minimum degree of uterine vascularization and score 4, maximum vascularization. Doppler velocimetry of right and left uterine arteries will be performed at the level of the uterine body in longitudinal section. With the use of colour Doppler, the gate will be positioned when a vessel with good colour signals could be identified. Subsequently, flow velocity waveforms were obtained with the pulsed-wave Doppler. Parameters of blood flow velocity such as systolic peak velocity (SPV), end-diastolic peak (EDV) and time-average maximum velocity (TAMAX), as well as hemodynamic parameters such as resistance index (RI), pulsatility index (PI) and systolic-diastolic ratio (S/D) will be calculated electronically. Uterine angiogenesis, as well as cellular proliferation and inflammation analysis will be performed through immunolocalization of VEGF and its receptor KDR (kinase insert domain-containing) and Flt-1 (fms-like tyrosinase kinase); and the nuclear antigen Ki-67 and cicloxigenase-2 inflammation biomarker, respectively. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
LIMA VEIGA, GISELE ALMEIDA; MIZIARA, RICARDO HENRIQUE; RAMOS ANGRIMANI, DANIEL SOUZA; PAPA, PAULA CARVALHO; COGLIATI, BRUNO; VANNUCCHI, CAMILA INFANTOSI. Cystic endometrial hyperplasia-pyometra syndrome in bitches: identification of hemodynamic, inflammatory, and cell proliferation changes. BIOLOGY OF REPRODUCTION, v. 96, n. 1, p. 58-69, . (12/22958-9)

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