Cardiac evaluation in bitches with sepsis due to cystic endometrial hyperplasia-pyometra complex

• Grant number: 12/02247-0
• Support Opportunities: Regular Research Grants
• Start date: June 01, 2012
• End date: May 31, 2014
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: Aparecido Antonio Camacho
• Grantee: Aparecido Antonio Camacho
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

Sepsis is defined as a systemic inflammatory response that occurs during infection. This syndrome represents a clinical problem of great importance due to its high prevalence and high mortality, especially when there is multiple organ dysfunction. In order to study the degree of cardiac dysfunction by canine patients will be evaluated 20 bitches, without distinction of breed, weighing up to 20 kg, who had sepsis due to the cystic endometrial hyperplasia pyometra complex. The animals will undergo for cardiovascular assessment by measurement of arterial blood pressure, conventional ECG and ambulatory electrocardiography (Holter) by one hour for observation of time-domain heart rate variability and also Doppler echocardiography to measure cardiac chambers sizes and determination of systolic, diastolic, and global myocardial function indexes. Blood samples will be collected to evaluate serum concentrations of tumor necrosis factor-alpha, interleukin 1, 6 e 10 and C-reactive protein, as well as biochemical analysis of serum alanine aminotransferase, alkaline phosphatase, urea, creatinine, total protein and albumin. After these procedures, animals will undergo for ovariohysterectomy. Fifteen and 30 days after surgery animals will be re-submitted to all procedures for evaluating cardiovascular function and blood tests. The results of the studied parameters will be subjected to normality test of residuals (Cramer-von Mises) and analysis of variance with subsequent Tukey post hoc test to compare means. Values will also be tested using multiple regression test. Statistical analyzes will be performed with a significance level of 5%. (AU)