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"Comparative evaluation laboratorial: antigenemia, N-PCR and qPCR to an early diagnosis of active HCMV infection and correlation with clinical manifestations"

Grant number: 11/03503-8
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): June 01, 2011
Effective date (End): November 30, 2012
Field of knowledge:Health Sciences - Medicine - Medical Clinics
Principal researcher:Sandra Cecília Botelho Costa
Grantee:Sílvia Renata Chagas Costa
Home Institution: Centro de Hematologia e Hemoterapia (HEMOCENTRO). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Tradução do português para inglêsHuman cytomegalovirus (HCMV) is a cosmopolitan virus, its prevalence is in Brazil, approximately 90% and 50% in developed countries. Transmission occurs through contact with infected body fluids and, when found in immunocompromised individuals, infection is much worse the greater the immunosuppression, since it may also affect the lungs to the central nervous system. Therefore, patients undergoing allogeneic hematopoietic stem cell allogeneic transplantation (HSCT) are potential victims of HCMV, with an incidence of infection after transplantation ranging from 32% to 90% with an average of 50% mainly in the first three months without antiviral treatment, and is the major cause of morbidity and mortality in these patients. To decrease this incidence, the Blood Center of Campinas, patients undergoing HSCT performed weekly collection of blood samples for performing N-PCR and antigenemia for monitoring possible active infection and, if necessary, initiate antiviral therapy (preemptive therapy). However, these tests are not standardized making it difficult to determine which one is most effective in early detection of active HCMV infection. This work aims to, by obtaining the result of laboratory tests and outpatient consultations record the records of patients undergoing HSCT, and the results obtained in the work of doctoral Renata Peres, to compare the laboratory techniques: antigenemia, N -PCR and qPCR for the diagnosis of active HCMV infection and its clinical manifestations.