Cellular and gene therapy in experimental fresh and cryopreserved ovary transplant

• Grant number: 10/17897-5
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: July 01, 2011
• End date: June 30, 2013
• Field of knowledge: Health Sciences - Medicine - Maternal and Child Health
• Principal Investigator: Edmund Chada Baracat
• Grantee: Luciana Lamarão Damous
• Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Cryopreserved ovary transplant has proved to be effective in the preservation of fertility in young patients who are submitted to cytotoxic treatments. Nevertheless, follicular depletion, after the transplant, limits future reproduction since the time lag spent in obtaining adequate perfusion of the transplanted tissue is an important factor of follicular survival and the functional longevity of the graft. Considering the imminent clinical applicability of the ovarian transplant in order to preserve female fertility and the necessity for techniques that enhance and optimize its use, this work aims at studying the effect of two kinds of treatments in fresh and cryopreserved ovary transplant in female rats - cellular therapy with pre-adipocytes and gene therapy with pre-adipocytes transfected with vascular endothelial growth factor - VEGF. Adult female rats were distributed in four experimental groups plus six control groups: First step: treatment with pre-adipocytes, and Second step: treatment with pre-adipocytes transfected with VEGF. Preadipocytes will be obtained from humane adipose tissue. Serum dosages of stradiol and progesterone will be performed before and after the transplant, besides daily vaginal smears until after thirty days after the transplant when euthanasia will occur. Morphological and histomorphometric analyses will be performed as well as immunohistochemical analysis of cellular proliferation (Ki-67), apoptosis (caspasis-3) and neoangiogenesis (VEGF -A). PCR study of neoangiogenesis, apoptosis inflammation and oxidative stress will also be performed.

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DAMOUS, LUCIANA LAMARAO; NAKAMUTA, JULIANA SANAJOTTI; SOARES-, JR., JOSE MARIA; ROSA MACIEL, GUSTAVO ARANTES; SIMOES, RICARDO DOS SANTOS; DE SOUZA MONTERO, EDNA FRASSON; KRIEGER, JOSE EDUARDO; BARACAT, EDMUND CHADA. Females transplanted with ovaries subjected to hypoxic preconditioning show impair of ovarian function. JOURNAL OF OVARIAN RESEARCH, v. 7, MAR 20 2014. (12/09469-9, 10/17897-5)

DAMOUS, LUCIANA LAMARAO; NAKAMUTA, JULIANA SANAJOTTI; TEOFILO SATURI DE CARVALHO, ANA ELISA; SOARES-, JR., JOSE MARIA; SIMOES, MANUEL DE JESUS; KRIEGER, JOSE EDUARDO; BARACAT, EDMUND C.. Adipose tissue-derived stem cell therapy in rat cryopreserved ovarian grafts. STEM CELL RESEARCH & THERAPY, v. 6, APR 15 2015. (12/09469-9, 10/17897-5)

LUCIANA LAMARAO DAMOUS; MARCOS EIJI SHIROMA; ANA ELISA TEOFILO SATURI DE CARVALHO; JOSE MARIA SOARES JR; JOSE EDUARDO KRIEGER; EDMUND C. BARACAT. Gene expression profile in experimental frozen-thawed ovarian grafts treated with scaffold-base delivery of adipose tissue-derived stem cells. Clinics, v. 77, 2022-08-08. (10/17897-5, 12/09469-9)

DAMOUS, LUCIANA LAMARAO; TEOFILO SATURI DE CARVALHO, ANA ELISA; NAKAMUTA, JULIANA SANAJOTTI; SHIROMA, MARCOS EIJI; SPOSATO LOUZADA, ANDRESSA CRISTINA; SOARES-, JR., JOSE MARIA; KRIEGER, JOSE EDUARDO; BARACAT, EDMUND C.. Cell-free therapy with the secretome of adipose tissue-derived stem cells in rats' frozen-thawed ovarian grafts. STEM CELL RESEARCH & THERAPY, v. 9, NOV 21 2018. (12/09469-9, 10/17897-5)

DAMOUS, LUCIANA LAMARAO; NAKAMUTA, JULIANA SANAJOTTI; TEOFILO SATURI DE CARVALHO, ANA ELISA; CARVALHO, KATIA CANDIDO; SOARES-, JR., JOSE MARIA; SIMOES, MANUEL DE JESUS; KRIEGER, JOSE EDUARDO; BARACAT, EDMUND C.. Scaffold-based delivery of adipose tissue-derived stem cells in rat frozen-thawed ovarian autografts: preliminary studies in a rat model. JOURNAL OF ASSISTED REPRODUCTION AND GENETICS, v. 32, n. 8, p. 1285-1294, AUG 2015. (12/09469-9, 10/17897-5)