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Comparative analysis of proteome of roots, stems and leaves of two clones E. grandis x E. camaldulensis resistant and susceptible to drought stress

Grant number: 10/17223-4
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): August 01, 2011
Effective date (End): February 28, 2013
Field of knowledge:Biological Sciences - Genetics - Plant Genetics
Principal Investigator:Carlos Alberto Labate
Grantee:Janaina de Santana Borges
Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil


The increasing demand for wood products in domestic and international market requires constant wood production, and the Eucalyptus genus is an alternative to meet this demand with high productivity and reduced production costs. The site selection for planting this genus requires studies related tocharacteristics of adaptability of the species to be used. For regions with drought stress, for example, a drought resistant clone or species must be selectedMany authors have shown the potential of Eucalyptus camaldulensis and hybrids of this species and E. grandis for production in arid regions of Brazil. The scarcity of water in the soil causes stomatal closure in plants and may block the influx of CO2 into the leaves reducing the accumulation of fotoassimilates. The adaptability of a species to a particular environment can berelated to many factors, such as its genome and proteome. The cells of an organism have the same genome, but perform different functions and display diverse morphologies as result of differences in gene expression pattern and expressed proteins. That illustrates the importance of studying not only gene sequences, but also their expression in order to understand theirbiological functions.The goal of this work is to study the proteins expressed in twohybrid clones of E. grandis x E. camaldulensis, one susceptible and another resistant to drought stress.The proteins profile and phosphoproteins expressed in leaves,stems and roots of young plants (6 months) submited to drought stress will be characterized. The proteins will be separated by bi-dimensional electrophoresis and MudPIT and sequenced by ESI-MS/MS. (AU)

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Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
BORGES, Janaina de Santana. Comparative analysis of roots´s proteome and metabolome, of two clones E. grandis x E. camaldulensis tolerant and susceptible under drought stress conditions. 2013. Master's Dissertation - Universidade de São Paulo (USP). Escola Superior de Agricultura Luiz de Queiroz (ESALA/BC) Piracicaba.

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