Comparative analysis of roots´s proteome and metabolome, of two clones E. grandis x E. camaldulensis tolerant and susceptible under drought stress conditions

Author(s)

Janaina de Santana Borges

• Document type: Master's Dissertation
• Press: Piracicaba.
• Institution: Universidade de São Paulo (USP). Escola Superior de Agricultura Luiz de Queiroz (ESALA/BC)
• Defense date: 2013-06-05

Examining board members

Carlos Alberto Labate; Camila Caldana; Claudia Barros Monteiro Vitorello

• Advisor: Carlos Alberto Labate

Abstract

The increasing demand for wood products in the domestic and international markets requires constant wood production and the Eucalyptus genus is an alternative to meet this demand. The site selection for planting this genus requires studies related to characteristics of adaptability of the species to be used. For regions with drought stress, for example, a drought resistant clone or species must be selected. Many authors have shown the potential of Eucalyptus camaldulensis and hybrids of this species with E. grandis for production in arid regions of Brazil. The adaptation of a species to a particular environment may be related to many genetic features which have an impact, for example, on the proteome and metabolome of this species. The cells in an organism have the same genome, but exhibit the most varied functions and morphologies, which are related to differences in the expression pattern of proteins and metabolites of those cells. The areas of proteomics and metabolomics can assist in the understanding the biological processes and supply an overview about plants status at any given time and in response to certain conditions / environmental stresses. The aim of this work is to perform a comparative analysis of the roots´s proteome and metabolome of two E. grandis x E. camaldulensis clones, one tolerant and another susceptible to drought, after the individuals have been subjected to different water regimes, 100% of field capacity for the control plants and 30% for the treated ones. The results showed the presence of proteins related to drought stress and the presence of secondary metabolites differentially expressed in the control when compared to treatment plants. This research has an innovative feature to be one of the first works involving proteomics and metabolomics studies of eucalyptus roots under water stress. New researches related to this field are welcome and may help to identify genes tolerant to drought stress that may be used in the future for genetic engineering plants. (AU)

• FAPESP's process: 10/17223-4 - Comparative analysis of proteome of roots, stems and leaves of two clones E. grandis x E. camaldulensis resistant and susceptible to drought stress
• Grantee: Janaina de Santana Borges
• Support Opportunities: Scholarships in Brazil - Master