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Viability and fertility of chilled bull semen with or without addition of cryoprotectant

Grant number: 11/05040-5
Support type:Scholarships in Brazil - Master
Effective date (Start): August 01, 2011
Effective date (End): July 31, 2013
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Reproduction
Principal researcher:José Antonio Dell'Aqua Junior
Grantee:Patricia de Mello Papa
Home Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil


The use of chilled bull semen has gained significant importance in the last decade, either in the national and international level. In Brazil, this technology has grown significantly over the past three years due to the increased practice of fixed time artificial insemination (FTAI). As in Brazil, Australia and New Zealand shows great potential in AI programs for beef or dairy cattle. The main advantages related to chilled semen are lower sperm concentration per insemination dose, optimization of genetically superior bulls, lower cost of storage and its practical use in AI. These factors together reduce animal production costs, allowing the implementation and dissemination of AI and FTAI techniques in several national livestock production systems. Thus, the aim of the present study is to compare the viability and fertility of chilled bovine semen diluted in BotuBov® with and without the addition of glycerol according the patterns of motility by computadorized method - CASA, plasma membrane integrity, and acrosomal and mitochondrial potential by flow cytometry in samples stored at 0 and 24 hours of storage in different refrigeration systems and after freezing. The fertility will be evaluated after laboratory experiments through fixed time artificial insemination. Data analysis will help to elucidate not only the viability of commercial use of chilled bull semen, but the real influence of its quality on conception rates through FTAI programs, comparing it with frozen samples. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PAPA, PATRICIA M.; MAZIERO, ROSIARA D.; GUASTI, PRISCILLA N.; JUNQUEIRA, CASSIO R.; FREITAS-DELL'AQUA, CAMILA P.; PAPA, FREDERICO O.; VIANNA, FELIPE P.; ALVARENGA, MARCO A.; CRESPILHO, ANDRE M.; DELL'AQUA, JR., JOSE ANTONIO. Effect of glycerol on the viability and fertility of cooled bovine semen. Theriogenology, v. 83, n. 1, p. 107-113, . (11/05040-5)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
PAPA, Patricia de Mello. Efeito da incorporação do glicerol sobre a viabilidade e fertilidade do sêmen bovino refrigerado. 2013. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária e Zootecnia. Botucatu Botucatu.

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