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Characterization of the gene coding for the alpha subunit of the channel chlorine (GluClalpha) possibly associated with resistance to macrocyclic lactones in Cochliomyia hominivorax (Diptera: Calliphoridae)

Grant number: 11/01030-5
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): August 01, 2011
Effective date (End): February 28, 2013
Field of knowledge:Biological Sciences - Genetics - Animal Genetics
Principal Investigator:Ana Maria Lima de Azeredo-Espin
Grantee:Alberto Moura Mendes Lopes
Host Institution: Centro de Biologia Molecular e Engenharia Genética (CBMEG). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil


Brazil has the largest commercial cattle herd in the world, being one of the areas of income generation for the most important country. Infestations by ectoparasites and endoparasites are among the main problems that lead to decreased productivity. Among them, Cochliomyia hominivorax (Coquerel), known as fly-pest stands out as one of the main causes of myiasis, generating losses of approximately US$ 150 million per year to the country. Systemic products such as macrocyclic lactones, activating chloride channel (GluCl±) have been extensively used as prevention and treatment of infestations by C. hominivorax in several parasites, and severe or prolonged use of macrolactones has led to the emergence of resistance to such products. The identification of molecular mechanisms of resistance to macrolactones in other species indicates the occurrence of possible changes in the chloride channel associated with resistance in C. hominivorax. Using sequences of the chloride channel from other species, previously deposited in the database, and sequences obtained in the characterization of the transcriptome of C. hominivorax, the objective of this project is to amplify and sequence the coding region of the chloride channel gene (GluCl±) in C. hominivorax. Furthermore, the sequences will be compared to individuals susceptible and resistant to macrolactones order to interpret(s) mutation(s) that gives resistance to such products and, consequently, the molecular diagnosis of resistance. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MENDES LOPES, ALBERTO MOURA; DE CARVALHO, RENATO ASSIS; LIMA DE AZEREDO-ESPIN, ANA MARIA. Glutamate-gated chloride channel subunit cDNA sequencing of Cochliomyia hominivorax (Diptera: Calliphoridae): cDNA variants and polymorphisms. INVERTEBRATE NEUROSCIENCE, v. 14, n. 2, p. 137-146, . (11/01030-5)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
LOPES, Alberto Moura Mendes. Characterization of the gene encoding the alpha subunit of the chloride channel (GluCl alpha) possibly associated with resistance to macrocyclic lactones in Cochliomyia hominivorax(Diptera: Calliphoridae). 2013. Master's Dissertation - Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia Campinas, SP.

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