Effects of leucine and branched-chaim amino acids supplementation on resistance exercise-induced muscle damage and inflammation: a randomized, double-blind, and placebo-controlled study

• Grant number: 11/04690-6
• Support Opportunities: Scholarships in Brazil - Master
• Start date: August 01, 2011
• End date: March 31, 2013
• Field of knowledge: Health Sciences - Nutrition
• Principal Investigator: Antonio Herbert Lancha Junior
• Grantee: Claudia Ribeiro da Luz
• Host Institution: Escola de Educação Física e Esporte (EEFE). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

This study aims to evaluate the effects of branched chain amino acids (BCAA) supplementation on resistance exercise-induced muscle damage and inflammation. A cross-sectional, randomized, double-blind, and placebo-controlled study will be conducted. Thirty male, sedentary, and 18-30 years aged subjects will be divided into three groups: BCAA, leucine and placebo (n=10 for each group). Subjects will be randomized according to the isometric strenght. The groups will be submmitted to eccentric exercise protocol consisting of 120 maximal eccentric contractions of the quadriceps muscle performed using an isokinetic dynamometer at angular velocity of 60°/s. The supplementation protocol will consist of 3.5 g of leucine + 3.5 g of alanine for leucine group, 3.5 g of leucine+ 1.7 g of isoleucine + 1.7 g of valine for BCAA group, and 7.0 g of alanine for placebo group. After strength test familiarization, subjects will receive nutritional intervention and consume the supplements during 4 days following the intervention. At baseline, immediately before, after and during 4 days of intervention the volunteers will carry blood collection and assessment of muscle pain. Measurement of isometric strenght will be measured at baseline, after 48 and 96 hours after the exercise session. Additionally, volunteers will undergo muscle biopsies for protein expression analysis and immunohistochemistry at baseline and at the end of the study. Blood samples will be used to measure plasma levels of insulin, glucose, cytokines (IL-1², IL-4, IL-6, IL-10, TNF-±, IFN-³), creatine kinase (CK), and C-reactive protein; biopsies will be used for protein expression of cytokines described and immunohistochemistry to verify inflammatory infiltrates in the muscle fibers. With this, we intend to assess the effectiveness of BCAA and leucine supplementation in attenuating exercise-induced acute muscle damage and inflammation and the possible cellular mechanisms involved in order to propose a possible nutritional therapy.OuvirLer foneticamenteDicionário - Ver dicionário detalhado (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

NICASTRO, HUMBERTO; ZANCHI, NELO EIDY; DA LUZ, CLAUDIA RIBEIRO; SEIXAS CHAVES, DANIELA FOJO; LANCHA, JR., ANTONIO HERBERT. An Experimental Model for Resistance Exercise in Rodents. JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY, 2012. (10/07062-3, 11/04690-6)

NICASTRO, HUMBERTO; ZANCHI, NELO E.; DA LUZ, CLAUDIA R.; DE MORAES, WILSON M. A. M.; RAMONA, PAMELLA; DE SIQUEIRA FILHO, MARIO A.; CHAVES, DANIELA F. S.; MEDEIROS, ALESSANDRA; BRUM, PATRICIA C.; DARDEVET, DOMINIQUE; et al. Effects of leucine supplementation and resistance exercise on dexamethasone-induced muscle atrophy and insulin resistance in rats. NUTRITION, v. 28, n. 4, p. 465-471, APR 2012. (10/10852-6, 10/07062-3, 11/04690-6)

NICASTRO, H.; ZANCHI, N. E.; DA LUZ, C. R.; LANCHA, JR., A. H.. Functional and morphological effects of resistance exercise on disuse-induced skeletal muscle atrophy. Brazilian Journal of Medical and Biological Research, v. 44, n. 11, p. 1070-1079, NOV 2011. (10/07062-3, 11/04690-6)

NICASTRO, HUMBERTO; DA LUZ, CLAUDIA R.; CHAVES, DANIELA F. S.; DAS NEVES, WILLIAM; VALENTE, KEROLYN S.; LANCHA, JR., ANTONIO H.. Leucine supplementation combined with resistance exercise improves the plasma lipid profile of dexamethasone-treated rats. LIPIDS IN HEALTH AND DISEASE, v. 11, JAN 11 2012. (10/07062-3, 11/04690-6)

NICASTRO, HUMBERTO; DA LUZ, CLAUDIA RIBEIRO; SEIXAS CHAVES, DANIELA FOJO; GRASSMANN BECHARA, LUIZ ROBERTO; VOLTARELLI, VANESSA AZEVEDO; ROGERO, MARCELO MACEDO; LANCHA, ANTONIO HERBERT, JR.. Does Branched-Chain Amino Acids Supplementation Modulate Skeletal Muscle Remodeling through Inflammation Modulation? Possible Mechanisms of Action. JOURNAL OF NUTRITION AND METABOLISM, v. 2012, p. 10-pg., 2012-01-01. (11/04690-6, 10/07062-3)

NICASTRO, HUMBERTO; ZANCHI, NELO EIDY; DA LUZ, CLAUDIA RIBEIRO; SEIXAS CHAVES, DANIELA FOJO; LANCHA, ANTONIO HERBERT, JR.. An Experimental Model for Resistance Exercise in Rodents. JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY, v. N/A, p. 7-pg., 2012-01-01. (10/07062-3, 11/04690-6)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

LUZ, Claudia Ribeiro da. Effects of leucine and branched chain amino acids supplementation associated with resistance exercise on the Akt / mTOR signaling pathway: a randomized, double-blind, placebo-controlled. 2013. Master's Dissertation - Universidade de São Paulo (USP). Escola de Educação Física e Esportes (EEFE/BT) São Paulo.