Development of techniques for transmission electron microscopy for the diagnosis of Avipoxvirus in commercial breeding birds and Ecological Parks of the State of São Paulo.

• Grant number: 11/13374-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: October 01, 2011
• End date: September 30, 2012
• Field of knowledge: Biological Sciences - Microbiology - Biology and Physiology of Microorganisms
• Principal Investigator: Marcia Helena Braga Catroxo
• Grantee: Rodrigo Barbosa de Souza
• Host Institution: Instituto Biológico (IB). Agência Paulista de Tecnologia dos Agronegócios (APTA). Secretaria de Agricultura e Abastecimento (São Paulo - Estado). São Paulo , SP, Brazil

Abstract

The avian pox, caused by a poxvirus, a member of the Poxviridae family and the Avipoxvirus genus, is an acute and highly contagious disease of birds, that infects both the commercial and free-living, causing major damage to the creations and creators. The disease is characterized by three main forms, cutaneous, diphtheric and septicemic, but the forms of tumors and runny nose may also occur. This project aims to detect the presence of avipoxvírus in samples of skin lesions, scabs and oral and ocular swabs of birds from breeding and Ecological Parks of the State of São Paulo, Brazil. In the Electron Microscopy Laboratory techniques will be processed for transmission electron microscopy in negative staining, immunoelectron microscopy, immunocytochemistry (immunolabeling with colloidal gold particles) and the resin embedding to contribute to subsequent routine screening of these viruses in birds, determining risk areas in the State of São Paulo and collaborating in this way with the National Agribusiness Poultry, giving the foundation health programs.