Ticks are ectoparasites arthropods, hematophagous of mandatory throughout the ontogenetic cycle, classified within the Subclass Acari, comprising three families: Ixodidae, Argasidae and Nutalliellidae. Ixodidae and Argasidae families are large in numbers of species and geographic distribution, parasitizing vertebrates as birds, amphibians, reptiles and mammals. These ectoparasites have great importance shown by direct injuries that cause to the host and by transmitting to these pathogens. The subfamily Amblyomminae shelters the single genus Amblyomma, being that many of the species in this genus occur in Brazil. However, the phylogeny of this group is still controversial and there are insufficient data to enhance the characteristics of the species considered basal, as well as the Brazilian species in general. In this sense, morphological studies comparing the internal systems of males, mainly the cells (somatic and germ) of its reproductive system, although still scarce, has provided important information when focus the phylogenetic relationships among the individuals of the same family. Similarly, the search for effective control methods and non-polluting against ticks have currently been target of many studies, such as the use of esters of ricinoleic acid from castor oil, which have been shown to promising acaricides in lab tests with Rhipicephalus sanguineus. Given the importance of ticks of the subfamily Amblyomminae and the lack of phylogenetic information about this group, this project aims to confirm and bring new data complementing the phylogenetic tree that already exists for this subfamily, making for both morphological and molecular techniques use comparative mitochondrial rDNA of Brazilian and Australian species of the genus Amblyomma, verifying concurrently the action of esters of ricinoleic acid from castor oil as an acaricide on the embryogenesis of Brazilian species A. cajennense and A. triste, that has great medical and veterinary importance in Brazil. To do this, will be used Histological techniques, such as scanning electron microscopy, transmission electron microcscopy and Laser Confocal microscopy to the description of the internal anatomy and morphology of the male reproductive systems and the process of embryogenesis (via study of embryonic development of eggs obtained from females postures), as well as make use of DNA sequencing techniques of these same species for obtaining data that can be compared.
News published in Agência FAPESP Newsletter about the scholarship: