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Comparative spermiogenesis and embryogenesis between ticks species of the genus Amblyomma (Acari: Ixodidae: phylogenetic and natural chemical control aspects

Grant number: 12/02384-8
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): June 01, 2012
Effective date (End): May 31, 2016
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Pathology
Principal Investigator:Maria Izabel Souza Camargo
Grantee:Bruno Rodrigues Sampieri
Home Institution: Instituto de Biociências (IB). Universidade Estadual Paulista (UNESP). Campus de Rio Claro. Rio Claro , SP, Brazil

Abstract

Ticks are ectoparasites arthropods, hematophagous of mandatory throughout the ontogenetic cycle, classified within the Subclass Acari, comprising three families: Ixodidae, Argasidae and Nutalliellidae. Ixodidae and Argasidae families are large in numbers of species and geographic distribution, parasitizing vertebrates as birds, amphibians, reptiles and mammals. These ectoparasites have great importance shown by direct injuries that cause to the host and by transmitting to these pathogens. The subfamily Amblyomminae shelters the single genus Amblyomma, being that many of the species in this genus occur in Brazil. However, the phylogeny of this group is still controversial and there are insufficient data to enhance the characteristics of the species considered basal, as well as the Brazilian species in general. In this sense, morphological studies comparing the internal systems of males, mainly the cells (somatic and germ) of its reproductive system, although still scarce, has provided important information when focus the phylogenetic relationships among the individuals of the same family. Similarly, the search for effective control methods and non-polluting against ticks have currently been target of many studies, such as the use of esters of ricinoleic acid from castor oil, which have been shown to promising acaricides in lab tests with Rhipicephalus sanguineus. Given the importance of ticks of the subfamily Amblyomminae and the lack of phylogenetic information about this group, this project aims to confirm and bring new data complementing the phylogenetic tree that already exists for this subfamily, making for both morphological and molecular techniques use comparative mitochondrial rDNA of Brazilian and Australian species of the genus Amblyomma, verifying concurrently the action of esters of ricinoleic acid from castor oil as an acaricide on the embryogenesis of Brazilian species A. cajennense and A. triste, that has great medical and veterinary importance in Brazil. To do this, will be used Histological techniques, such as scanning electron microscopy, transmission electron microcscopy and Laser Confocal microscopy to the description of the internal anatomy and morphology of the male reproductive systems and the process of embryogenesis (via study of embryonic development of eggs obtained from females postures), as well as make use of DNA sequencing techniques of these same species for obtaining data that can be compared.

Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ARVEY RIVERA-PAEZ, FREDY; SAMPIERI, BRUNO RODRIGUES; LABRUNA, MARCELO BAHIA; MATOS, RENATA DA SILVA; MARTINS, THIAGO FERNANDES; CAMARGO-MATHIAS, MARIA IZABEL. Comparative analysis of germ cells and DNA of the genus Amblyomma: adding new data on Amblyomma maculatum and Amblyomma ovale species (Acari: Ixodidae). Parasitology Research, v. 116, n. 11, p. 2883-2892, NOV 2017. Web of Science Citations: 1.
SAMPIERI, BRUNO RODRIGUES; CALLIGARIS, IZABELA BRAGIAO; MATOS, RENATA DA SILVA; RIVERA PAEZ, FREDY ARVEY; BUENO, ODAIR CORREA; CAMARGO-MATHIAS, MARIA IZABEL. Comparative analysis of spermatids of Rhipicephalus sanguineus sensu lato (Ixodidae) and Ornithodoros rostratus ticks (Argasidae): morphophysiology aimed at systematics. Parasitology Research, v. 115, n. 2, p. 735-743, FEB 2016. Web of Science Citations: 1.
SAMPIERI, BRUNO RODRIGUES; LABRUNA, MARCELO BAHIA; BUENO, ODAIR CORREA; CAMARGO-MATHIAS, MARIA IZABEL. Dynamics of cell and tissue genesis in the male reproductive system of ticks (Acari: Ixodidae) Amblyomma cajennese (Fabricius, 1787) and Amblyomma aureolatum (Pallas, 1772): a comparative analysis. Parasitology Research, v. 113, n. 4, p. 1511-1519, APR 2014. Web of Science Citations: 4.
SAMPIERI, B. R.; FURQUIM, K. C. S.; NUNES, P. H.; CAMARGO-MATHIAS, M. I. Rhipicephalus sanguineus (Acari: Ixodidae) female ticks exposed to castor oil (Ricinus communis): an ultrastructural overview. Parasitology Research, v. 112, n. 2, p. 611-619, FEB 2013. Web of Science Citations: 2.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
SAMPIERI, Bruno Rodrigues. Morfologia e desenvolvimento do sistema reprodutor masculino de carrapatos do gênero Amblyomma (Acari, Ixodidae) : uma análise comparativa. 2016. 104 f. Doctoral Thesis - Universidade Estadual Paulista "Júlio de Mesquita Filho" Instituto de Biociências (Campus de Rio Claro)..

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