Response of cutaneous lymphoma to chemotherapic therapy in face of MDR-1 gene expression

• Grant number: 11/11191-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: September 01, 2011
• End date: August 31, 2012
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: Luiz Henrique de Araújo Machado
• Grantee: Fúlvia Bueno de Souza
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Because of the improvement in domestic animal healthcare and the progresses occurred in veterinary medicine, it is now necessary to study the diseases more deeply and to establish clinical and laboratorial routines to provide correct diagnosis and to help in the evaluation of prognosis. In order to elucidate doubts in veterinary oncology, the present study intends to evaluate the efficiency of apoptosis and proliferation index and of MDR-1 gene expression before and after chemotherapy on the establishment of prognosis in cases of canine cutaneous lymphoma. Dogs with diagnosis of cutaneous lymphoma will be submitted to clinical staging and anatomical classification by histopathology. After these results, samples will be submitted to immunofenotyping with anti-CD3, anti-CD79a, anti-caspase-3 and anti-MIB-1 antibodies and expression of MDR-1 will be verified by PCR. All the dogs will be submitted to chemotherapy and evaluation of response to this association and the results.