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Reactivity to the proteins glycoprotein-P, p53 and MDM2 in lymphomatous dogs

Grant number: 13/20422-7
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): January 01, 2014
Effective date (End): December 31, 2014
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Pathology
Principal researcher:Áureo Evangelista Santana
Grantee:Elis Bueno dos Santos
Home Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

The lymphoma is a malignant neoplasia of lymphoid tissue that primarily affects the lymph nodes or other solid visceral organs such as the liver or spleen, corresponding to approximately 90% of the neoplasias of the hematopoietic system in dogs. It is the type of cancer that better responds to chemotherapy, and, without treatment, many dogs die in four to six weeks. However, tumors such as breast, ovarian and lymphoma may recur after an initial response to chemotherapy and become resistant to a large quantity of drugs. Many cellular mechanisms that show the drug resistance have been characterized, among which are the P-glycoprotein (P-gp), associated in the reduction of intracellular drug concentrations, the expression of the p53 gene, regulator of apoptosis and MDM2, responsible for decreasing p53 levels by his degradation. Therefore, the aim of this study is to evaluate the expression of P-gp, p53 and MDM2 protein in lymph nodes from 20 dogs with multicentric lymphoma at the time of diagnosis, through immunohistochemistry. The slides will be evaluated through scores by immunostaining in each lesion, considering the extent and intensity of staining.