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Measure of anti-apoptotic and apoptotic factors related to infection of monocytes with CDV

Grant number: 11/13507-0
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): September 01, 2011
Effective date (End): October 31, 2013
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
Principal researcher:Tereza Cristina Cardoso da Silva
Grantee:Natielle Rodrigues Wajima
Home Institution: Faculdade de Medicina Veterinária (FMVA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil
Associated scholarship(s):12/21356-5 - Phylogenetic characterization of canine distemper virus isolates from naturally infected dogs in Brazil, BE.EP.IC


Canine distemper virus (CDV) may induce multifocal demyelination in the central nervous system of infecteddogs. The present work investigated apoptosis in white and gray matter (granular layer) in the cerebellum of naturallyinfected dogs by the analysis of the expression of the pro-apoptotic antigens caspase - 2 and - 3 , b(terminaldeoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL-staining) positivity, annexin-Vimmunodetection, and the presence of the anti-apoptotic antigens, BCl-2 and p53. Cerebellum specimens were obtainedfrom the Laboratory of Animal Pathology, from 1995 to 2009, and the 5-¼m thick fragments were stained both withhematoxylin-eosin and Shorr. All samples were diagnosed as positive for CDV genome by reverse transcriptasepolymerase chain reaction targeting the nucleocapsid gene. The anti-apoptotic pathways evidenced in this study wereBCl-2 and p53 proteins that were intensively detected in cerebellum of CDV positive slides (40-80% of labeledcells/mm2). In addition, the apoptosis markers annexin-V and TUNEL are directly correlated among the same samples(80 and 40% of labeled cells, respectively). This is the first description of p53 and annexin-V expression, characterizedas anti-apoptotic and apoptotic proteins, involvement in canine natural cases of CDV infections.

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