Phylogenetic characterization of canine distemper virus isolates from naturally infected dogs in Brazil

• Grant number: 12/21356-5
• Support Opportunities: Scholarships abroad - Research Internship - Scientific Initiation
• Start date: January 28, 2013
• End date: February 27, 2013
• Field of knowledge: Agronomical Sciences - Veterinary Medicine
• Principal Investigator: Tereza Cristina Cardoso da Silva
• Grantee: Natielle Rodrigues Wajima
• Supervisor: Yanina Panzera Crespo
• Host Institution: Faculdade de Medicina Veterinária (FMVA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil
• Institution abroad: Universidad de la República (UDELAR), Uruguay
• Associated to the scholarship: 11/13507-0 - Measure of anti-apoptotic and apoptotic factors related to infection of monocytes with CDV, BP.IC

Abstract

The canine distemper virus is the etiologic agent of a multisystem infection that affects different species of carnivores and is responsible for a major disease affected by dogs. It is closely related to measles virus and the "rinderpest" virus, belonging to the family Paramyxoviridae, genus Morbillivirus. It is enveloped with a single RNA strand of negative polarity, including biological properties of infecting different cell types: epithelial cells, mesenchymal, hematopoietic and neuroendocrine of different organs and / or tissues. Recent data have shown a worldwide increase in the incidence of the disease, including the population of vaccinated dogs, which requires analysis of circulating strains. The hemagglutinin gene (H), which encodes the major antigenic viral protein, has been widely used to determine the degree of genetic variability and to associate CDVs worldwide strains of different current. Further studies showed that there are two different strains circulating in Argentina and Uruguay that are prevalent strain in Europe, called Europe 1 lineage and South America 2 lineage, these previously found only in wild animals. The present study aims at sequencing the hemagglutinin gene (H) for the analysis of divergent amino acids present in the strains found in naturally infected animals in the region of São Paulo, Brazil. (AU)