Role Of matrix metalloproteinases 2 and 9 inhibitors (TIMPs 1 and 2) in the transmissible venereal tumor (TVT).

• Grant number: 11/08290-2
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: November 01, 2011
• End date: February 28, 2014
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Pathology
• Principal Investigator: Renee Laufer Amorim
• Grantee: Rafael Ricardo Huppes
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

ABSTRACTTransmissible Venereal Tumor (TVT) is a major neoplasms of stray dogs, usually affecting the vulva and penis, this is a cancer that is spread through intercourse, due to abrasion of the penis to the vulva. The TVT is a cancer that has rarely metastases, may occur in approximately 5 to 17% of cases, with major sites to regional lymph nodes and abdominal cavity. This study aims to evaluate the gene expression of MMPs 2 and 9 and their inhibitors (TIMP: tissue inhibitors of metalloproteinase), TIMPs 1 and 2, by the methods of qRT-PCR and immunohistochemical in TVTs and correlates them with clinical features and histopathological tumor. To do so will be collected from 30 dogs TVTs. Tumors are classified as lymphoid and plasmacytoid according to Gaspar (2005), clinical data will be recorded as location, size, number of nodules, presence or absence of metastases, response to treatment and possible relapse and compared to gene and protein expression each of the biomarkers, statistical test of analysis of variance. It will also compare the gene expression of MMP-2 and 9 with the gene (TIMP: tissue inhibitors of metalloproteinase), TIMPs 1 and 2. Therefore be expected to assess some aspects of transmission and possible mechanism of tumor metastasis, and corroborate the understanding of metastatic processes in cancer.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RR HUPPES; M CARVALHO; TMMR FERREIRA; YT GRANJA-SALCEDO; RAR USCATEGUI; JLC CASTRO; PM JOSIANI; AB DE NARDI; RL AMORIM. Can the ATM and TP53 levels influence the low metastasis potential of canine TVT?. ARCHIVOS DE MEDICINA VETERINARIA, v. 48, n. 1, p. 107-111, 2016-00-00. (11/08290-2)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

HUPPES, Rafael Ricardo. Expressão gênica de MMP-2 e 9, TIMP-1 e 2, ATM, TP53, VEGF, COX-2 e CDH-1 no TVT canino. 2014. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal Jaboticabal.