Evaluation in vivo of the effect of corticosteroids and ventilation in oxide nitric pathway on the expression of VEGF and its pulmonary receptors after fetal tracheal occlusion in the congenital diaphragmatic hernia in rats

• Grant number: 11/12587-0
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: October 01, 2011
• End date: September 30, 2014
• Field of knowledge: Health Sciences - Medicine - Surgery
• Principal Investigator: Lourenço Sbragia Neto
• Grantee: Frances Lilian Lanhellas Gonçalves
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

A congenital diaphragmatic hernia (CDH) is a defect that usually occurs on the left side for not closing the posterolateral foramen of the diaphragmatic during fetal life and allows the passage of abdominal organs into the thorax.The presence of abdominal organs into the thorax acts as a lesion compressing the lungs and its normal development and as a consequence cause a maturation and pulmonary hypoplasia. To promote lung growth in utero and neonatal mortality decrease of HDC, new techniques have been perfected and among them is fetal tracheal occlusion (TO) which enables the acceleration of lung maturity. Allied to TO, there are further treatments such as ventilation, which may also offer improved compliance of the body and corticosteroids, which are known anti-inflammatory actions, and act on the permeability of membranes, enzymatic synthesis and transport of the mucosa. The administration in the prenatal period has beneficial effects on lung growth. The evaluation of these treatments can be performed by studying of the nitric oxide (NO) pathway, which is a essencial mediator of endothelial cell migration and VEGF (Vascular Endothelial Growth Factor). VEGF is the main stimulator of angiogenesis that acts to induce mitosis and migration of vascular endothelial cells. In addition to promoting angiogenesis and neovascularization is also a survival factor for newly formed blood vessels. Therefore, assessing the relationship between treatment with corticosteroids and ventilation of the experimental HDC with the production of NO through the isoforms and their interaction with TO on the development of the pulmonary vasculature, especially on the receptors of VEGF, can bring benefits for understanding the pathophysiology and treatment of disease. (AU)

Scientific publications

(18)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FRANCES LILIAN LANHELLAS GONÇALVES; FÁBIO SANTANA DE OLIVEIRA; AUGUSTO FREDERICO SCHMIDT; LUÍS ANTÔNIO VIOLIN DIAS PEREIRA; RODRIGO MELO GALLINDO; REBECA LOPES FIGUEIRA; LOURENÇO SBRAGIA. Efeito do nitrofen na fase final do desenvolvimento da musculatura do diafragma em ratos. Acta Cirurgica Brasileira, v. 28, p. 13-18, 2013-00-00. (11/12587-0, 11/00794-1)

GONCALVES, FRANCES L. L.; BUENO, MARCIA P.; SCHMIDT, AUGUSTO F.; FIGUEIRA, REBECA L.; SBRAGIA, LOURENCO. Treatment of bowel in experimental gastroschisis with a nitric oxide donor. American Journal of Obstetrics and Gynecology, v. 212, n. 3, MAR 2015. (11/12587-0, 11/00794-1)

SCHMIDT, AUGUSTO F.; GONCALVES, FRANCES L. L.; REGIS, ALINE C.; GALLINDO, RODRIGO M.; SBRAGIA, LOURENCO. Prenatal retinoic acid improves lung vascularization and VEGF expression in CDH rat. American Journal of Obstetrics and Gynecology, v. 207, n. 1, JUL 2012. (11/12587-0, 11/00794-1)

SCHMIDT, AUGUSTO FREDERICO; LANHELLAS GONCALVES, FRANCES LILIAN; FIGUEIRA, REBECA LOPES; SCORLETTI, FEDERICO; PEIRO, JOSE LUIS; SBRAGIA, LOURENCO. Combined antenatal therapy with retinoic acid and tracheal occlusion in a rat model of congenital diaphragmatic hernia. PEDIATRIC SURGERY INTERNATIONAL, v. 32, n. 6, p. 591-598, JUN 2016. (11/12587-0, 11/00794-1, 14/02519-6)

LANHELLAS GONCALVES, FRANCES LILIAN; BITTENCOURT, DANIEL GUIMARAES; VELLOSO, LICIO AUGUSTO; SCHMIDT, AUGUSTO FREDERICO; GALLINDO, RODRIGO MELO; SBRAGIA, LOURENCO. Corticosteroid effect upon intestinal and hepatic interleukin profile in a gastroschisis rat model. Acta Cirurgica Brasileira, v. 28, n. 1, p. 8-12, 2013. (11/12587-0, 11/00794-1)

LANHELLAS GONCALVES, FRANCES LILIAN; DE OLIVEIRA, FABIO SANTANA; SCHMIDT, AUGUSTO FREDERICO; VIOLIN DIAS PEREIRA, LUIS ANTONIO; GALLINDO, RODRIGO MELO; FIGUEIRA, REBECA LOPES; SBRAGIA, LOURENCO. Effect of nitrofen in the final stages of development of the diaphragm muscle in rats. Acta Cirurgica Brasileira, v. 28, n. 1, p. 13-18, 2013. (11/12587-0, 11/00794-1)

LANHELLAS GONCALVES, FRANCES LILIAN; GALLINDO, RODRIGO MELO; MANGUEIRA SOARES, LUCAS MANOEL; FIGUEIRA, REBECA LOPES; PERECIM VOLPE, FABIO ANTONIO; PEREIRA-DA-SILVA, MAURCIO ANDRE; SBRAGIA, LOURENCO. Validation of protocol of experimental necrotizing enterocolitis in rats and the pitfalls during the procedure. Acta Cirurgica Brasileira, v. 28, n. 1, p. 19-25, 2013. (11/12587-0, 11/00794-1)

L. SBRAGIA; A.C.C. NASSR; F.L.L. GONÇALVES; A.F. SCHMIDT; C.C. ZULIANI; P.V. GARCIA; R.M. GALLINDO; L.A.V. PEREIRA. VEGF receptor expression decreases during lung development in congenital diaphragmatic hernia induced by nitrofen. Brazilian Journal of Medical and Biological Research, v. 47, n. 2, p. 171-178, 2014-02-00. (11/12587-0, 11/00794-1)

MANSO, P. H.; FIGUEIRA, R. L.; PRADO, C. M.; GONCALVES, F. L.; SIMOES, A. L. B.; RAMOS, S. G.; SBRAGIA, L.. Early neonatal echocardiographic findings in an experimental rabbit model of congenital diaphragmatic hernia. Brazilian Journal of Medical and Biological Research, v. 48, n. 3, p. 234-239, MAR 2015. (11/12587-0, 11/00794-1)

GALLINDO, RODRIGO MELO; LANHELLAS GONCALVES, FRANCES LILIAN; FIGUEIRA, REBECA LOPES; VIOLIN DIAS PEREIRA, LUIS ANTONIO; BERTONCINI SIMOES, ANA LEDA; SCHMIDT, AUGUSTO FREDERICO; SBRAGIA, LOURENCO. Ventilation causes pulmonary vascular dilation and modulates the NOS and VEGF pathway on newborn rats with CDH. JOURNAL OF PEDIATRIC SURGERY, v. 50, n. 5, p. 842-848, MAY 2015. (11/12587-0, 11/00794-1)

FIGUEIRA, R. L.; GONCALVES, F. L.; PRADO, A. R.; RIBEIRO, M. C.; COSTA, K. M.; CASTRO E SILVA, O.; SBRAGIA, L.. Ventilation-induced changes correlate to pulmonary vascular response and VEGF, VEGFR-1/2, and eNOS expression in the rat model of postnatal hypoxia. Brazilian Journal of Medical and Biological Research, v. 51, n. 11, p. 8-pg., 2018-01-01. (12/09951-5, 11/12587-0)

FIGUEIRA, R. L.; GONCALVES, F. L.; PRADO, A. R.; RIBEIRO, M. C.; COSTA, K. M.; CASTRO E SILVA, O.; SBRAGIA, L.. Ventilation-induced changes correlate to pulmonary vascular response and VEGF, VEGFR-1/2, and eNOS expression in the rat model of postnatal hypoxia. Brazilian Journal of Medical and Biological Research, v. 51, n. 11, 2018. (12/09951-5, 11/12587-0)

FRANCES LILIAN LANHELLAS GONÇALVES; DANIEL GUIMARÃES BITTENCOURT; LÍCIO AUGUSTO VELLOSO; AUGUSTO FREDERICO SCHMIDT; RODRIGO MELO GALLINDO; LOURENÇO SBRAGIA. Efeito do corticoesteróide sobre o perfil das interleucinas intestinais e hepáticas no modelo de gastrosquise em ratos. Acta Cirurgica Brasileira, v. 28, p. 08-12, 2013-00-00. (11/12587-0, 11/00794-1)

FRANCES LILIAN LANHELLAS GONÇALVES; RODRIGO MELO GALLINDO; LUCAS MANOEL MANGUEIRA SOARES; REBECA LOPES FIGUEIRA; FÁBIO ANTÔNIO PERECIM VOLPE; MAURÍCIO ANDRÉ PEREIRA-DA-SILVA; LOURENÇO SBRAGIA. Validação do modelo de enterocolite necrotizante experimental em ratos e as armadilhas durante sua execução. Acta Cirurgica Brasileira, v. 28, p. 19-25, 2013-00-00. (11/12587-0, 11/00794-1)

R.L. FIGUEIRA; F.L. GONÇALVES; A.L. SIMÕES; C.A. BERNARDINO; L.S. LOPES; O. CASTRO E SILVA; L. SBRAGIA. Brain caspase-3 and intestinal FABP responses in preterm and term rats submitted to birth asphyxia. Brazilian Journal of Medical and Biological Research, v. 49, n. 7, 2016-06-23. (11/12587-0, 11/00794-1)

LANHELLAS GONCALVES, FRANCES LILIAN; FIGUEIRA, REBECA LOPES; SIMOES, ANA LEDA BERTONCINI; GALLINDO, RODRIGO MELO; COLEMAN, ALLAN; PEIRO, JOSE LUIS; SBRAGIA, LOURENCO. Effect of corticosteroids and lung ventilation in the VEGF and NO pathways in congenital diaphragmatic hernia in rats. PEDIATRIC SURGERY INTERNATIONAL, v. 30, n. 12, p. 1207-1215, DEC 2014. (11/12587-0, 11/00794-1)

LANHELLAS GONCALVES, FRANCES LILIAN; FIGUEIRA, REBECA LOPES; GALLINDO, RODRIGO MELO; BERTONCINI SIMOES, ANA LEDA; COLEMAN, ALAN; PEIRO, JOSE LUIS; SBRAGIA, LOURENCO. Tracheal occlusion and ventilation changes the nitric oxide pathway in congenital diaphragmatic hernia model. JOURNAL OF SURGICAL RESEARCH, v. 203, n. 2, p. 466-475, JUN 15 2016. (11/12587-0, 11/00794-1, 14/02519-6)

GALLINDO, RODRIGO MELO; LANHELLAS GONCALVES, FRANCES LILIAN; DE RESENDE BARRETO, CAROLINA TEIXEIRA; SANTOS SCHMIDT, AUGUSTO FREDERICO; VIOLIN DIAS PEREIRA, LUIS ANTONIO; SBRAGIA, LOURENCO. Evaluation of histological changes after tracheal occlusion at different gestational ages in a fetal rat model. Clinics, v. 68, n. 1, p. 59-63, 2013. (11/12587-0, 11/00794-1)