Immortalization of cell lines from xeroderma pigmentosum patients by expression of genes E6 and E7 of human papillomavirus.

• Grant number: 11/17777-2
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: October 01, 2011
• End date: September 30, 2012
• Field of knowledge: Biological Sciences - Genetics - Mutagenesis
• Principal Investigator: Carlos Frederico Martins Menck
• Grantee: Rachel Emy Straus Takahashi
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

This project aims the immortalizations of cell lines of xeroderma pigmentosum patients and their relatives with no clinical manifestations. The immortalization of these cell lines is going to be made by vector transduction and expression of genes E6 and E6 of HPV16. By this process we are going to be able to mantain these cell lines for indetermined time in culture, allowing us to make more studies of these cell lines. This project also aims the comparisson of UV-radiation sensitivity between transformed cells and primary cells to verify the consequences to the DNA rapair pathway caused by the interference of these genes in XP cells.