Ergogenic response of melatonin during the nadir and acrophase of spontaneous activity: consequences on IKK/Nf-kB via

• Grant number: 11/13226-1
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: March 01, 2012
• End date: February 28, 2015
• Field of knowledge: Health Sciences - Physical Education
• Principal Investigator: Claudio Alexandre Gobatto
• Grantee: Wladimir Rafael Beck
• Host Institution: Faculdade de Ciências Aplicadas (FCA). Universidade Estadual de Campinas (UNICAMP). Limeira , SP, Brazil

Abstract

Melatonin (n-acetyl-5-methoxytryptamine) is a hormone produced by the pineal gland that has various functions. Classically known to control circadian rhythms and sleep-wake cycle, has recently been investigated for its antioxidant role, to preserve glycogen stores through modulation of lipid and carbohydrate metabolism during exercise and by the inhibition of transcriptional nuclear factor kappa B (NF-kB),concomitantly decreasing the signaling cascade of inflammation and oxidative stress. Most studies in this area, especially when they involve physical activity, is fragile in some intervenient parameters, such as the effects of circadian rhythm, control of exercise intensity and time of application of experimental procedures. Nevertheless, the literature reports unidirectional experiments, without taking into account the various properties of this hormone. Given this context, the objective of this project is to determine the ergogenic capacity of melatonin in exercise continued until exhaustion in lactate minimum intensity (LMi), in two times of day (higher and lower spontaneous activity), identifying the possible effects of this hormone in the circadian rhythm of rats spontaneous activity (CRRSA) and via IKK / NF-kB activity . To determine the CRRSA will be used a gravimetric system via strain gages, which consists in analyze the force responses on the rats cage, generated by muscular action, independent of kinematic activity of the animals, with 24 hours of monitoring and acquisition frequency of 1000Hz. To determine the effort intensity will be used the lactate minimum test in tethered swimming system. The force increments and monitoring of propulsion response will be captured in 1000Hz. The animals will be divided into 16 groups to be isolated from the following effects: melatonin administration, performance (exercise until exhaustion in LMi) and two times of day (higher and lower spontaneous activity). The animals will be euthanized 1 or 24 hours after the performance, in a CO2 chamber. The blood will be collected by cardiac puncture to determine the melatonin concentration. Muscle and liver tissues samples will be extracted to determination of glycogen concentration and IKK/NF-kB activity.

Scientific publications

(10)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BECK, W.; GOBATTO, C.. Effect of high wavelengths low intensity light during dark period on physical exercise performance, biochemical and haematological parameters of swimming rats. ACTA PHYSIOLOGICA HUNGARICA, v. 103, n. 1, p. 112-119, MAR 2016. (10/13377-7, 12/20501-1, 11/13226-1)

BECK, W. R.; SCARIOT, P. P. M.; GOBATTO, C. A.. Melatonin is an Ergogenic Aid for Exhaustive Aerobic Exercise only during the Wakefulness Period. INTERNATIONAL JOURNAL OF SPORTS MEDICINE, v. 37, n. 1, p. 71-76, JAN 2016. (09/08535-5, 10/13377-7, 11/13226-1)

BECK, WLADIMIR RAFAEL; BOTEZELLI, JOSE DIEGO; PAULI, JOSE RODRIGO; ROPELLE, EDUARDO ROCHETE; GOBATTO, CLAUDIO ALEXANDRE. Melatonin Has An Ergogenic Effect But Does Not Prevent Inflammation and Damage In Exhaustive Exercise. SCIENTIFIC REPORTS, v. 5, DEC 16 2015. (09/08535-5, 12/20501-1, 11/13226-1)

BECK, W. R.; RIBEIRO, L. F. P.; SCARIOT, P. P. M.; DOS REIS, I. G. M.; GOBATTO, C. A.. Time of day effects on aerobic capacity, muscle glycogen content and performance assessment in swimming rats. SCIENCE & SPORTS, v. 29, n. 6, p. 319-323, DEC 2014. (10/13377-7, 11/13226-1)

WLADIMIR RAFAEL BECK; LUCAS DANTAS MAIA FORTE; CLAUDIO ALEXANDRE GOBATTO. EFEITOS DO HORÁRIO DO DIA, LUMINOSIDADE AMBIENTAL E EXERCÍCIO FÍSICO SOBRE PARÂMETROS INFLAMATÓRIOS E DE PERFORMANCE EM RATOS NADADORES. Rev. educ. fis. UEM, v. 26, n. 1, p. 97-106, 2015-03-00. (11/13226-1, 12/20501-1, 10/13377-7)

BECK, W. R.; SCARIOT, P. P. M.; GOBATTO, C. A.. Primary and secondary thrombocytosis induced by exercise and environmental luminosity. BRATISLAVA MEDICAL JOURNAL-BRATISLAVSKE LEKARSKE LISTY, v. 115, n. 10, p. 607-610, 2014. (10/13377-7, 11/13226-1)

BECK, W.; GOBATTO, C.. Effects of maximum intensity aerobic swimming exercise until exhaustion at different times of day on the hematological parameters in rats. ACTA PHYSIOLOGICA HUNGARICA, v. 100, n. 4, p. 427-434, DEC 2013. (10/13377-7, 11/13226-1)

BECK, WLADIMIR R.; CAMPESAN, YURI S.; GOBATTO, CLAUDIO A.. Validity and reliability of incremental test to determine the anaerobic threshold in swimming rats. INTERNATIONAL JOURNAL OF APPLIED EXERCISE PHYSIOLOGY, v. 4, n. 1, p. 25-33, APR 2015. (09/08535-5, 10/13377-7, 12/20501-1, 11/13226-1)

BECK, W.; GOBATTO, C.. Effect of high wavelengths low intensity light during dark period on physical exercise performance, biochemical and haematological parameters of swimming rats. PHYSIOLOGY INTERNATIONAL, v. 103, n. 1, p. 8-pg., 2016-03-01. (10/13377-7, 11/13226-1, 12/20501-1)

BECK, W. F.; DE ARAUJO, G. G.; MENEZES SCARIOT, P. P.; MASSELLI DOS REIS, I. G.; GOBATTO, C. A.. Time to exhaustion at anaerobic threshold in swimming rats: metabolic investigation. BRATISLAVA MEDICAL JOURNAL-BRATISLAVSKE LEKARSKE LISTY, v. 115, n. 10, p. 617-621, 2014. (10/13377-7, 11/13226-1)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

BECK, Wladimir Rafael. Ergogenic response of melatonin ar nadir and acrophase of spontaneous activity and its consequences on IKK/NF-kB pathway activity and muscle tissue damage. 2015. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Faculdade de Educação Física Campinas, SP.