Xanthomonas campestris pv. campestris: survival and detection of ecological niches in brassicas crop fields with occurrence of black rot

• Grant number: 11/18527-0
• Support type: Scholarships in Brazil - Post-Doctorate
• Effective date (Start): November 01, 2011
• Effective date (End): October 31, 2014
• Field of knowledge: Agronomical Sciences - Agronomy - Plant Health
• Principal researcher: Antonio Carlos Maringoni
• Grantee: Tadeu Antônio Fernandes da Silva Júnior
• Home Institution: Faculdade de Ciências Agronômicas (FCA). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated scholarship(s): 12/22521-0 - Xanthomonas campestris pv. campestris: survival and detection of ecological niches in brassicas crop fields with occurrence of black rot, BE.EP.PD

Abstract

Black rot, incited by Xanthomonas campestris pv. campestris (Xcc), is one of the main diseases of brassicas in many countries, leading to losses up to 60% in production. The knowledge of survival ecological niches of phytopathogenic bacteria is extremely important for the appropriate management of phytobacteriosis, once a small amount of survival inoculum between crop seasons can be enough to development of a new epidemic in the field. Through the use of an Xcc mutant resistant to 100 µg.mL-1 of rifampicin, the survival capacity of the bacteria will be evaluated in different assays. Xcc survival as free cells in the soil will be evaluated in four assays, under field conditions, in different times of the year. The bacteria survival capability in cauliflower crop debris will be determined under laboratory conditions, in samples incubated at different temperatures. The epiphytic, endophytic and rhizosphere colonization of crop plants and weeds by Xcc will be evaluated in assays under greenhouse conditions. Samplings in brassica crop fields with occurrence of black rot will be performed to verify the presence of Xcc in samples of soil, debris, crop plants and weeds. Bacterial colonies with similar characteristics to Xcc will be quantified, selected and submitted to characterization by differential Gram stain, starch hydrolysis, growth in YDC medium, pathogenicity and PCR.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SILVA JUNIOR, T. A. F.; SILVA, J. C.; GONCALVES, R. M.; SOMAN, J. M.; PASSOS, J. R. S.; MARINGONI, A. C. Survival of Xanthomonas campestris pv. campestris associated with soil and cauliflower crop debris under Brazilian conditions. European Journal of Plant Pathology, v. 156, n. 2 NOV 2019. Web of Science Citations: 0.

SILVA, J. C.; SILVA JUNIOR, T. A. F.; SOMAN, J. M.; TOMASINI, T. D.; SARTORI, M. M. P.; MARINGONI, A. C. Survival of Xanthomonas campestris pv. campestris in the phyllosphere and rhizosphere of weeds. PLANT PATHOLOGY, v. 66, n. 9, p. 1517-1526, DEC 2017. Web of Science Citations: 2.