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Xanthomonas campestris pv. campestris: survival and detection of ecological niches in brassicas crop fields with occurrence of black rot

Grant number: 12/22521-0
Support type:Scholarships abroad - Research Internship - Post-doctor
Effective date (Start): April 01, 2013
Effective date (End): March 31, 2014
Field of knowledge:Agronomical Sciences - Agronomy - Plant Health
Principal researcher:Antonio Carlos Maringoni
Grantee:Tadeu Antônio Fernandes da Silva Júnior
Supervisor abroad: Jan van der Wolf
Home Institution: Faculdade de Ciências Agronômicas (FCA). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
Research place: Wageningen University, Netherlands  
Associated to the scholarship:11/18527-0 - Xanthomonas campestris pv. campestris: survival and detection of ecological niches in brassicas crop fields with occurrence of black rot, BP.PD


Black rot, incited by Xanthomonas campestris pv. campestris (Xcc), is one of the main diseases of brassicas in many countries, leading to losses up to 60% in production. The knowledge of survival ecological niches of phytopathogenic bacteria is extremely important for the appropriate management of phytobacteriosis, once a small amount of survival inoculum between crop seasons can be enough to development of a new epidemic in the field. Through the use of an Xcc mutant resistant to 100 ¼g.mL-1 of rifampicin, the survival capacity of the bacteria will be evaluated in different assays. Xcc survival as free cells in the soil will be evaluated in four assays, under field conditions, in different times of the year, and in three assays under controlled conditions. The survivability of the bacteria in crop residues of cauliflower will be determined in three trials under field conditions during different times of the year. The epiphytic, entophytic and rhizosphere colonization of crop plants and weeds by Xcc will be evaluated in assays under greenhouse conditions. Samplings in brassica crop fields with occurrence of black rot will be performed to verify the presence of Xcc in weeds (rizosphere soil, phyloplane and endophytic colonization).Bacterial colonies with similar characteristics to Xcc will be quantified, selected and submitted to characterization by differential Gram stain, starch hydrolysis, growth in YDC medium, pathogenicity and PCR. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
VAN DER WOLF, JAN; KASTELEIN, PIETER; FERNANDES DA SILVA JUNIOR, TADEU ANTONIO; LELIS, FLAVIA VIEIRA; VAN DER ZOUWEN, PATRICIA. Colonization of siliques and seeds of rapid cycling Brassica oleracea plants by Xanthomonas campestris pv. campestris after spray-inoculation of flower clusters. European Journal of Plant Pathology, v. 154, n. 2, p. 445-461, JUN 2019. Web of Science Citations: 0.

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