Development and validation of new analytical methods for determination of anti-histamine in pharmaceutical dosage forms.

• Grant number: 11/17130-9
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: November 01, 2011
• End date: January 31, 2012
• Field of knowledge: Health Sciences - Pharmacy - Medicines Analysis and Control
• Principal Investigator: Anil Kumar Singh
• Grantee: Diogo Eiji Nakao Takano
• Host Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Antihistamines are widely used in clinical cases of allergic conditions where histamine is the main mediator, as in some chronic urticaria, seasonal and perennial rhinitis, allergic conjunctivitis and solar erythema. The expected effects of the anti-histamine on H1 receptor antagonists are different depending on the administered dose strength and active pharmaceutical ingredient used, however, most of them significantly decrease capillary permeability by blocking the vasodilatation caused by histamine, thus reducing the clinical manifestation of type I allergies. To ensure quality, safety and efficacy of these medications it is of outmost importance to quantitatively determine these substances in pharmaceutical dosage forms.In this project, the objective is to develop and validate new analytical methods using High performance liquid chromatography and UV Spectrophotometry that are efficient, cheep, simple, economical, accurate and precise and apply these methods for quantitative determination of desloratadine in pharmaceutical dosage forms.