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Effect of hydrogen sulfide (H2S) in the commissural NTS on the ventilatory and hemodynamic response to hypoxia and hypercapnia in normotensive and hypertensive animals

Grant number: 11/14779-4
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): May 01, 2012
Effective date (End): April 30, 2016
Field of knowledge:Biological Sciences - Physiology
Principal Investigator:Luiz Guilherme de Siqueira Branco
Grantee:João Paulo Jacob Sabino
Home Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Associated scholarship(s):14/12951-2 - Role endogenous of hydrogen sulphide (H2S) in the NTS on respiratory activity during hypoxia, BE.EP.PD

Abstract

Hypertension is a public health problem that affects a large portion of world population that in most cases don`t know that they have the disease. Hypertension has similarities with essential hypertension observed in Spontaneous Hypertensive Rats (SHR). Among the central regions involved with the increased sympathetic activity and blood pressure in SHR is commissural NTS (cNTS), which receives baroreceptor and chemoreceptor afferents and where the presence of the enzyme cystathionine beta-synthase (CBS) has been documented. This enzyme endogenously forms H2S which has been reported as the third member of the gaseous neurotransmitter, after nitric oxide (NO) and carbon monoxide (CO). No reports exist about the role of H2S in the cNTS, and its relationship with cardiovascular and respiratory responses to hypoxia and hypercapnia in hypertensive and normotensive animals. Therefore, we aim to shed light into the neurochemical control of cardiovascular and respiratory systems, assessing the putative participation of H2S in the cNTS, mediating physiological responses to hypoxia and hypercapnia of normo- and hypertensive rats. For this purpose will be run four experimental protocols, assessing: 1) the effects of micro-injections in the NTS, the CBS inhibitor (AOAA) and donor of H2S (Na2S) in pulmonary ventilation, blood pressure and heart rate during normocapnia and normoxia; 2) the effects of micro-injections in the NTS, the inhibitor CBS (AOAA) and donor of H2S (Na2S) in pulmonary ventilation, blood pressure and heart rate in response to hypercapnia; 3) the effects of micro-injections in the NTS, the inhibitor of the enzyme CBS (AOAA) and donor of H2S (Na2S) in pulmonary ventilation, blood pressure and heart rate in response to hypoxia, and 4) the comparative analysis of the expression of the enzyme CBS the cNTS between hypertensive and normotensive animals. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SABINO, JOAO PAULO J.; SORIANO, RENATO N.; SANTOS, BRUNA M.; DONATTI, ALBERTO F.; FERNANDEZ, RODRIGO R.; DA SILVA, GLAUBER S. F.; BRANCO, LUIZ G. S. Central administration of aminooxyacetate, an inhibitor of H2S production, affects thermoregulatory but not cardiovascular and ventilatory responses to hypercapnia in spontaneously hypertensive rats. Respiratory Physiology & Neurobiology, v. 263, p. 38-46, MAY 2019. Web of Science Citations: 0.
SABINO, JOAO PAULO J.; SORIANO, RENATO N.; DONATTI, ALBERTO F.; FERNANDEZ, RODRIGO RESTREPO; KWIATKOSKI, MARCELO; FRANCESCATO, HELOISA D. C.; COIMBRA, TEREZILA M.; BRANCO, LUIZ G. S. Involvement of endogenous central hydrogen sulfide (H2S) in hypoxia-induced hypothermia in spontaneously hypertensive rats. Canadian Journal of Physiology and Pharmacology, v. 95, n. 2, p. 157-162, FEB 2017. Web of Science Citations: 1.
SABINO, JOAO PAULO J.; ARIZA TRASLAVINA, GUILLERMO A.; BRANCO, LUIZ G. S. Role of central hydrogen sulfide on ventilatory and cardiovascular responses to hypoxia in spontaneous hypertensive rats. Respiratory Physiology & Neurobiology, v. 231, p. 21-27, SEP 2016. Web of Science Citations: 4.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.