The effect of using phytotherapics and propolis in physicochemical properties, antimicrobial and biocompatibility of MTA

• Grant number: 11/18272-1
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: February 01, 2012
• End date: January 31, 2015
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Marco Antonio Hungaro Duarte
• Grantee: Bruno Cavalini Cavenago
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil

Abstract

The aim of this study will be evaluate the physicochemical properties, biological and antimicrobial of white MTA mixed with the extracts of Arctium lappa L., Casearia sylvestris SW and propolis. The cements will be divided into six groups, and white MTA mixed with: aqueous extract of Arctium lappa L (G1), 80% distilled water + 20% propylene glycol extract of Arctium lappa L (G2), aqueous extract of Casearia sylvestris Sw (G3), 80% distilled water + 20% propylene glycol extract of Casearia sylvestris Sw (G4), 80% distilled water + 20% propylene glycol extract of propolis (G5) and distilled water (control-G6). To determine the setting time, the standard adopted will be ASTM C266/2008. The flow test will be accomplished according the ISO 6876/2001 specifications. The solubility will be determined volumetrically by microcomputed tomography. In chemical tests to evaluate the pH and calcium release, the materials will be placed in retrograde cavities in acrylic teeth and embedded in and ultrapure water, keeping for 3 hours, 1, 3, 7 and 15 days. This water will be measured for pH and calcium released by the atomic absorption spectrophotometer. To evaluate the antimicrobial effect will be applied direct contact method (Enterococcus faecalis and Candida albicans) to quantifying the colony forming units. Also will be performed the evaluation of intra-tubular decontamination segments of root canals of bovine teeth evaluated by confocal laser scanning microscopy. For the biocompatibility test will be used 162 rats divided into six groups and subdivided into three periods (15, 30 and 60). Each animal will receive two subcutaneous and one alveolar implant (polyethylene tube with cement). After animals death, the samples of subcutaneous and alveolar tissue will be submitted to histologically process to obtain a 5 µm thickness sections and stained with hematoxylin and eosin, the specimens will be proceeded to descriptive and quantitative microscopic analysis. According to the histological results, samples of the alveolar tissue will be processed to analyze the cytokine production by ELISA. Data from each test will be tested for normality by D'Agostino and Pearson, to determine the appropriate statistical test, always with a significance level of 5%. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CAVENAGO, BRUNO CAVALINI; DEL CARPIO-PEROCHENA, ALDO ENRIQUE; AMOROSO-SILVA, PABLO ANDRES; ALCALDE, MURILO PRIORI; FERNANDES, SAMUEL LUCAS; VIVAN, RODRIGO RICCI; HUNGARO DUARTE, MARCO ANTONIO. Evaluation of Mesial Root Canals of Mandibular Molars Obturated with Gutta-Percha and Resilon Techniques. EUROPEAN ENDODONTIC JOURNAL, v. 2, JUN 30 2017. (11/18272-1)

CAVENAGO, B. C.; ORDINOLA-ZAPATA, R.; DUARTE, M. A. H.; DEL CARPIO-PEROCHENA, A. E.; VILLAS-BOAS, M. H.; MARCIANO, M. A.; BRAMANTE, C. M.; MORAES, I. G.. Efficacy of xylene and passive ultrasonic irrigation on remaining root filling material during retreatment of anatomically complex teeth. International Endodontic Journal, v. 47, n. 11, p. 1078-1083, NOV 2014. (11/18272-1, 10/16072-2, 10/16002-4)

SANT'ANNA JUNIOR, ARNALDO; CAVENAGO, BRUNO CAVALINI; ORDINOLA-ZAPATA, RONALD; DE-DEUS, GUSTAVO; BRAMANTE, CLOVIS MONTEIRO; HUNGARO DUARTE, MARCO ANTONIO. The Effect of Larger Apical Preparations in the Danger Zone of Lower Molars Prepared Using the Mtwo and Reciproc Systems. JOURNAL OF ENDODONTICS, v. 40, n. 11, p. 1855-1859, NOV 2014. (11/18272-1, 12/06738-9)

CAVENAGO, BRUNO CAVALINI; DEL CARPIO-PEROCHENA, ALDO ENRIQUE; ORDINOLA-ZAPATA, RONALD; ESTRELA, CARLOS; GARLET, GUSTAVO POMPERMAIER; TANOMARU-FILHO, MARIO; WECKWERTH, PAULO HENRIQUE; DE ANDRADE, FLAVIANA BOMBARDA; HUNGARO DUARTE, MARCO ANTONIO. Effect of Using Different Vehicles on the Physicochemical, Antimicrobial, and Biological Properties of White Mineral Trioxide Aggregate. JOURNAL OF ENDODONTICS, v. 43, n. 5, p. 779-786, MAY 2017. (11/18272-1)

CAVENAGO, B. C.; PEREIRA, T. C.; DUARTE, M. A. H.; ORDINOLA-ZAPATA, R.; MARCIANO, M. A.; BRAMANTE, C. M.; BERNARDINELI, N.. Influence of powder-to-water ratio on radiopacity, setting time, pH, calcium ion release and a micro-CT volumetric solubility of white mineral trioxide aggregate. International Endodontic Journal, v. 47, n. 2, p. 120-126, FEB 2014. (11/18272-1, 10/16072-2)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

CAVENAGO, Bruno Cavalini. The effect of the use of phytotherapics and propolis in physicochemical properties, antimicrobial and biocompatibility of MTA. 2015. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Odontologia de Bauru (FOB/SDB) Bauru.