Photobiomodulation on regulatory myogenic factors expression during skeletal muscle repair following cryolesion in rats

• Grant number: 11/04452-8
• Support Opportunities: Scholarships in Brazil - Master
• Start date: March 01, 2012
• End date: December 31, 2012
• Field of knowledge: Health Sciences - Physiotherapy and Occupational Therapy
• Principal Investigator: Raquel Agnelli Mesquita Ferrari
• Grantee: Agnelo Neves Alves
• Host Institution: Universidade Nove de Julho (UNINOVE). Campus Memorial. São Paulo , SP, Brazil

Abstract

There is considerable interest in establishing therapies that provide a better, faster muscle repair process. A growing body of evidence suggests that low-level laser therapy (LLLT) promotes skeletal muscle regeneration, but its effectiveness in the treatment of injury remains questionable and its therapeutic potential is not fully established. The aim of the present study is to evaluate the effects of LLL on muscle repair and the expression of myogenic regulatory factors (MyoD, Myf5, myogenin and MRF4) following cryoinjury in rat skeletal muscle. Ninety one Wistar rats were divided into five groups: 1) control; 2) sham; 3) cryoinjured without treatment; 4) cryoinjured and LLLT; 5) non-injured and LLLT. The laser irradiation will be performed on the injured region using the GaAlAs laser (780nm) with a beam spot of 0.4 cm2, an output power of 40mW, energy density of 10J/cm2 for 10 seconds. After sacrifice, the Tibialis anterior muscle will be removed for the isolation of total RNA and analysis of myogenic regulatory factors using real-time PCR. Furthermore, muscle samples will be used for immunohistochemical regulatory myogenic factors analysis. The results will be statistically analyzed using ANOVA/Dunnet to determine differences between the control and treated groups. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ALVES, A. N.; FERNANDES, K. P. S.; MELO, C. A. V.; YAMAGUCHI, R. Y.; FRANCA, C. M.; TEIXEIRA, D. F.; BUSSADORI, S. K.; NUNES, F. D.; MESQUITA-FERRARI, R. A.. Modulating effect of low level-laser therapy on fibrosis in the repair process of the tibialis anterior muscle in rats. Lasers in Medical Science, v. 29, n. 2, SI, p. 813-821, MAR 2014. (11/04452-8, 11/17638-2)

ALVES, A. N.; FERNANDES, K. P. S.; MELO, C. A. V.; YAMAGUCHI, R. Y.; FRANCA, C. M.; TEIXEIRA, D. F.; BUSSADORI, S. K.; NUNES, F. D.; MESQUITA-FERRARI, R. A.. Modulating effect of low level-laser therapy on fibrosis in the repair process of the tibialis anterior muscle in rats. Lasers in Medical Science, v. 29, n. 2, p. 9-pg., 2014-03-01. (11/04452-8, 11/17638-2)

ALVES, AGNELO NEVES; RIBEIRO, BEATRIZ GUIMARES; SANTOS FERNANDES, KRISTIANNE PORTA; COSTA SOUZA, NADHIA HELENA; ROCHA, LILIA ALVES; NUNES, FABIO DAUMAS; BUSSADORI, SANDRA KALIL; MESQUITA-FERRARI, RAQUEL AGNELLI. Comparative effects of low-level laser therapy pre- and post-injury on mRNA expression of MyoD, myogenin, and IL-6 during the skeletal muscle repair. Lasers in Medical Science, v. 31, n. 4, p. 679-685, MAY 2016. (14/12381-1, 13/21540-3, 12/11461-6, 11/04452-8, 11/17638-2)

FRANCA, CRISTIANE MIRANDA; SANTANA, CRISTIANO DE LOURA; TAKAHASHI, CAMILA BORIN; ALVES, AGNELO NEVES; DE SOUZA MERNICK, ANA PAULA; SANTOS FERNANDES, KRISTIANNE PORTA; TEIXEIRA DA SILVA, DANIELA DE FATIMA; BUSSADORI, SANDRA KALIL; MESQUITA-FERRARI, RAQUEL AGNELLI. Effect of laser therapy on skeletal muscle repair process in diabetic rats. Lasers in Medical Science, v. 28, n. 5, p. 1331-1338, SEP 2013. (11/04452-8, 11/20867-3, 11/17638-2)

SANTOS FERNANDES, KRISTIANNE PORTA; ALVES, AGNELO NEVES; NUNES, FABIO DAUMAS; COSTA SOUZA, NADHIA HELENA; SILVA, JR., JOSE ANTONIO; BUSSADORI, SANDRA KALIL; MESQUITA FERRARI, RAQUEL AGNELLI. Effect of photobiomodulation on expression of IL-1 beta in skeletal muscle following acute injury. Lasers in Medical Science, v. 28, n. 3, p. 1043-1046, MAY 2013. (11/04452-8, 11/17638-2)