Molecular analysis of enteropathogenic Escherichia coli (EPEC) isolated from animals and humans.

• Grant number: 11/07579-9
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: February 01, 2012
• End date: December 31, 2014
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal Investigator: Fernando Antonio de Avila
• Grantee: Livia Gerbasi Beraldo
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

Enteropathogenic Escherichia coli (EPEC) is a category of E. coli interest in public health, since it is responsible for causing diarrhea, especially in children in developing countries. The aims of this project is determine the presence of related genes of LEE and non-LEE region of EPEC isolates from animals (buffaloes, pigs and sheep) and humans. Also will be studied cultures of Escherichia coli isolated from human feces unidentified in MacConkey agar plates, provided by the hospital clinical laboratory clinics from USP in Ribeirão Preto - SP. The strains from animal and human will be tested by PCR screening to detect the eae gene. Following the screening will be best characterized by PCR for the presence of espB genes (E2348/69), espB (EO26), espB (EDL933), espD, espD (EDL933), espF, pEAF, tir±, tir², tir³, nleA , nleB, nleE, and nleF nleH1-2. Additionally, subtyping will be done eae gene, which will be subtyped in ±, ², ³, º, ¶, ´, Ã, ¹. In addition, there will be a differentiation of subtypes ± 1, ± 2, ²1, ²2 and ³1, ³ 2 by PCR-RFLP method. EPEC isolates will be tested for antimicrobial susceptibility testing, and serological determination of serogroups. In addition, epidemiological link is established by PFGE of the isolates by PCR and phylogenetic analysis for Chua, and YjaA TspE4C2. The detection of virulence genes in the region LEE and non LEE of EPEC can show whether animals and humans can share the same genetic profiles. In addition, the detection of genes statistically associated with diarrhea in EPEC can show whether isolated belonging to the animal studies have zoonotic potential.

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

BERALDO, Livia Gerbasi. Análise molecular de estirpes de Escherichia coli enteropatogênica (EPEC) isoladas de animais e humanos. 2015. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal Jaboticabal.