Effect of temperature on the myogenic factors expression in skeletal muscle of pacu (Piaractus mesopotamicus)

• Grant number: 11/14538-7
• Support Opportunities: Scholarships in Brazil - Master
• Start date: March 01, 2012
• End date: August 31, 2013
• Field of knowledge: Agronomical Sciences - Fishery Resources and Fishery Engineering - Aquaculture
• Principal Investigator: Maeli Dal Pai
• Grantee: Tassiana Gutierrez de Paula
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Pacu fish has excellent features, such as fast growing, that promote the success of his intensive farming. Muscle growth in fish occurs by hypertrophy and/or hyperplasia involving satellite cells proliferation and differentiation; these processes are regulated by the expression of myogenic regulatory factors and myostatin. The aim of this study is to evaluate the influence of different temperatures of pacu rearing on MyoD, myogenin, myostatin and Pax-7 expression at different growth stages. Juveniles (1.5 g weight) will be distributed in tanks containing water and maintained at 25ºC, 30°C and at room temperature (six replicates for each group), under distinct recirculation systems, during the whole experiment. The samples collection will be performed at the beginning of the experiment and at 15, 30, 45 and 60 rearing days (n=24 animals for each group). The animals will be anesthetized, sacrificed and white muscle samples from dorsal region collected and frozen in liquid nitrogen for molecular and morphological analysis. Histological sections obtained in cryostat will be submitted to hematoxylin-eosin stain in order to perform muscle fibers morphology and morphometry analysis. The quantitative gene expression analysis of MyoD, myogenin and myostatin will be performed by Real Time Polymerase Chain Reaction after Reverse Transcription (RT-qPCR). Detection of MyoD, myogenin, myostatin and Pax-7 proteins and the count of marked nuclei will be analyzed by immunofluorescence technique. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE PAULA, TASSIANA GUTIERREZ; ALVES DE ALMEIDA, FERNANDA LOSI; CARANI, FERNANDA REGINA; VECHETTI-JUNIOR, IVAN JOSE; PADOVANI, CARLOS ROBERTO; SIMOES SALOMAO, RONDINELLE ARTHUR; MARECO, EDSON ASSUNCAO; DOS SANTOS, VANDER BRUNO; DAL-PAI-SILVA, MAELI. Rearing temperature induces changes in muscle growth and gene expression in juvenile pacu (Piaractus mesopotamicus). COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, v. 169, p. 31-37, MAR 2014. (11/14538-7)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

PAULA, Tassiana Gutierrez de. Efeito da temperatura sobre a expressão de fatores miogênicos no músculo estriado esquelético do pacu (Piaractus mesopotamicus). 2013. Master's Dissertation - Universidade Estadual Paulista (Unesp). Instituto de Biociências. Botucatu Botucatu.