Adhesion, differentiation and cellular proliferation of pre-osteoblast cultured on an laser irradiated titanium surface

• Grant number: 11/16351-1
• Support Opportunities: Scholarships in Brazil - Master
• Start date: March 01, 2012
• End date: March 31, 2013
• Field of knowledge: Health Sciences - Dentistry - Dental Materials
• Principal Investigator: Carlos Alberto de Souza Costa
• Grantee: Eduardo Mariscal Muñoz
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

Titanium is a widely used as a material for substitution of anatomic structures, being considered inside odontology as the most common material employed in cases of bone-integrated implants. It has been described that the osseo integration, requisite for a good functionality of implants, is a slowly process, sequentially characterized for steps of adhesion, differentiation and cellular proliferation, as well as for apposition and mineralization of matrix deposited for osteoblasts. Thus how the implant technic application, the bone quality and surface treatment of titanium have a direct influence on osseo integration process. Therefore, the objective of this work is to evaluate, in vitro, if the modification of the titanium surface with a high energy laser, could improve those biological processes of proliferation, differentiation, and metabolical activity on mouse calvarial pre-osteoblast. To perform this, primary cultured cells metabolism are going to be analyzed (MTT test), their phenotypes markers (qPCR), enzymatic activity of alkaline phosphatase and mineralization nodule formation (Alizarin-red) too. The numeric data obtained from this proposed experimental protocol, is going to be analyzed by a two fixed criteria variance analysis, according to characteristics of the combined data of dependent variant. Scientific data gotten with this study, will be truly relevant to knowledge in the area in which this investigation fits. Those because, to accelerate the osseo-integration process means to reduce waiting time to a safety apply of functional load over titanium implants.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MARISCAL-MUNOZ, EDUARDO; COSTA, CARLOS A. S.; TAVARES, HEWERSON S.; BIANCHI, JONAS; HEBLING, JOSIMERI; MACHADO, JOAO P. B.; LERNER, ULF H.; SOUZA, PEDRO P. C.. Osteoblast differentiation is enhanced by a nano-to-micro hybrid titanium surface created by Yb:YAG laser irradiation. CLINICAL ORAL INVESTIGATIONS, v. 20, n. 3, p. 503-511, APR 2016. (11/16351-1, 12/14912-9)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MUÑOZ, Eduardo Mariscal. Diferenciação de osteoblastos cultivados sobre superfícies de titânio modificados por irradiação com laser Yb: YAG pulsado de alta potencia. 2013. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araraquara Araraquara.