Identification and Quantification of Oxysterols in Atheromas and Plasma of Individuals With Peripheral Artery Occlusive Disease Submitted to Amputation Surgery

• Grant number: 11/16477-5
• Support Opportunities: Scholarships in Brazil - Master
• Start date: March 01, 2012
• End date: February 28, 2014
• Field of knowledge: Health Sciences - Medicine - Pathological Anatomy and Clinical Pathology
• Principal Investigator: Andrei Carvalho Sposito
• Grantee: Vitor Wilson de Moura Virginio
• Host Institution: Faculdade de Ciências Médicas (FCM). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Atherosclerosis has reached humans for thousand of years and nowadays is the major cause of cardiovascular disease (CVD) in the western civilization. The atheroma can be formed in several vascular vessels and present different composition accord to its location in the body. Basically the atheroma begins with an endothelial dysfunction leading to an inflammation processes and occlusion of the artery. Several lipids are known to be involved in this event, especially oxysterols class, involved in inflammation, immunological and homeostasis and cholesterol metabolism. Approximately one-third of the global death occurs duo to the CVD, and the major risk factors are smoking, dyslipidemias, inflammation and calcification of the vessels. These factors contribute to the limbs amputation due to occlusive arterial disease. The main aim of this project is to identify and quantify the oxysterols (24-, 25-, 27-hydroxycholesterol and 7-ketocholesterol) present in atheroma and plasma of 10 individuals submitted to amputation due to peripheral artery disease (PAD) and compare with the control group, about 13 individuals., using the technique of mass spectrometry coupled to gas chromatography. We also will study the possible relation between the oxysterols present in the atheroma and plasma with other variables, such as plasmatic proteins, clinical data and biochemical assays, using statistics tools, trying to find possible biomarkers in plasma.

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

VIRGINIO, Vitor Wilson de Moura. Identification and quantification of oxysterols in atheromas and plasma of individuals with occlusive peripheral arterial disease submitted to amputation surgery. 2014. Master's Dissertation - Universidade Estadual de Campinas (UNICAMP). Faculdade de Ciências Médicas Campinas, SP.