|Support type:||Scholarships in Brazil - Master|
|Effective date (Start):||March 01, 2012|
|Effective date (End):||September 30, 2013|
|Field of knowledge:||Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery|
|Principal researcher:||Fernando Jose Benesi|
|Grantee:||Carolina de Lara Shecaira|
|Home Institution:||Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil|
The birth of a calf, as well as other mammals, is marked by the passage of a sterile medium to a contaminated environment, without a fully developed immune system so that functionally, also sindesmocorial the placenta does not allow the passage of antibodies during pregnancy need to supply the newborn colostrum to confer immunity in early life. In addition to leukocytes has colostrum immunoglobulins, and cytokines that appear to participate in the establishment of neonatal immunity. Despite the potential importance of these components of colostrum, there are few studies evaluating the transfer of cytokines from colostrum and its role in neonatal immunity, which is why this project has the following objectives: to measure the concentrations of IL-1 beta, IL- 6, TNF-alpha and IFN-gamma present in the serum of newborn Holstein calves and evaluate the transfer and interference of the source of bovine colostrum and its offer, or otherwise, about the concentrations of these cytokines in serum of calves during the ten first days after birth. For that will be used 15 newborn calves born to normal delivery, shared equally by three experimental groups (n = 5), each with five animals: G1-receive 2 liters of fresh colostrum from their mothers, G2-receive 2 liters of colostrum from the "pool" of frozen colostrum-G3 and will be fed only milk. The first feeding will be provided within six hours after the birth of calves, and the next feedings of milk in a volume equivalent to 10% of body weight from neonates, divided into two daily meals. For the evaluation of cytokines present in the serum of calves, blood samples will be made during the first ten days of life after birth (pn), the following times: T0 (before the first intake of colostrum - G1 and G2, or first milk intake-G3), T1 (1-2 days pn), T2 (3-4 days pn), T3 (5-6 days pn) and T4 (7-8 days pn) and T5 (9-10 days pn).