Influence of oxidative damage in bovine sperm quality of different ages.

• Grant number: 11/16614-2
• Support Opportunities: Scholarships in Brazil - Master
• Start date: March 01, 2012
• End date: May 31, 2013
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: Marion Burkhardt de Koivisto
• Grantee: Juliane Teramachi Trevizan
• Host Institution: Faculdade de Medicina Veterinária (FMVA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

It is known that high levels of reactive oxygen species (ROS) in semen reduce the fertilizing potential of sperm cells. This study will investigate whether the oxidative damage, caused in bovine spermatozoa, varies with age and if the methods are effective in assessing oxidative stress for this species. For that 32 animals kept in an artificial insemination center will be distributed equally into four groups: group 1 from 20 to 24 months, group 2 from 59 to 72 months, group 3 from 84 to 112 months and group 4 from134 to 172 months. The animals will be collected twice weekly by artificial vagina completing three ejaculates per bull. The ejaculates will be frozen in accordance with the established standards of the artificial insemination center. The frozen semen will be evaluated for physical parameters (motility, vigor, concentration and morphology), production of ROS (superoxide anion) evaluated by DHE fluorochrome and Sytox ® Green, lipid peroxidation through the probe BODIPY 581/591 C11 and production DNA damage promoted by reactive oxygen species using the 8 - oxoguanina test.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

TREVIZAN, JULIANE TERAMACHI; CARREIRA, JANAINA TORRES; CARVALHO, ISADORA RESENDE; KIPPER, BRUNA HELENA; NAGATA, WALTER BERTEQUINE; VENTUROLI PERRI, SILVIA HELENA; FRANCO OLIVEIRA, MARIA EMILIA; PIERUCCI, JULIA CESTARI; DE KOIVISTO, MARION BURKHARDT. Does lipid peroxidation and oxidative DNA damage differ in Cheok rot cryopreserved semen samples from young, adult and aged Nellore bulls?. Animal Reproduction Science, v. 195, p. 8-15, AUG 2018. (11/16614-2)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

TREVIZAN, Juliane Teramachi. Influência dos danos oxidativos na qualidade espermática em bovinos de diferentes idades. 2013. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal Jaboticabal.