Genotyping of Giardia duodenalis: detection of mixed infections and genetic recombination in samples of human origin

• Grant number: 11/13472-2
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: March 01, 2012
• End date: February 28, 2015
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal Investigator: Rodrigo Martins Soares
• Grantee: Juliana Martins Aguiar
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Giardia duodenalis is a protozoan of worldwide distribution responsible for causing enteric infections in a wide variety of mammals, including humans. It is considered a species complex, in which the isolates can be classified into seven distinct genetic groupings (assemblages A, B, C, D, E, F, G), but morphologically indistinguishable. The genetic groups of G. duodenalis have different spectra of hosts. Two phenomena have been frequent in molecular studies: (i) occurrence of heterogeneous gene sequences, characterized by the presence of polymorphic sites, when the genes are amplified and sequenced with primers no specific assemblage and (ii) divergence between loci when different assemblages are marked by distinct genetic markers used in the same sample. Allelic sequence heterozygosis, genetic recombination and mixed infections are the explanations for such events; however, the only way to evaluate the real participation of these phenomena in samples of G. duodenalis would be through the analysis of a single Giardia cyst. To date no work of this nature was performed. In view of this lack, this project's main objective is isolation of cysts of G. duodenalis using the technique of micro-manipulation and subsequent genotyping by real-time PCR technique. The results to be obtained should bring contribution to knowledge of genetic and taxonomic of G. duodenalis, as well as the epidemiological knowledge of giardiasis.

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

JULIANA MARTINS AGUIAR; SHEILA OLIVEIRA SILVA; VALDIR AZEVEDO DOS SANTOS; SUELI AKEMI TANIWAKI; TRICIA MARIA FERREIRA DE SOUSA OLIVEIRA; HELENA LAGE FERREIRA; LARA BORGES KEID; FÁBIO GREGORI; RODRIGO MARTINS SOARES. Evidence of heterozygosity and recombinant alleles in single cysts of Giardia duodenalis. Revista Brasileira de Parasitologia Veterinária, n. ahead, p. 0-0, 2016. (11/13472-2)

AGUIAR, JULIANA MARTINS; SILVA, SHEILA OLIVEIRA; DOS SANTOS, VALDIR AZEVEDO; TANIWAKI, SUELI AKEMI; FERREIRA DE SOUSA OLIVEIRA, TRICIA MARIA; FERREIRA, HELENA LAGE; KEID, LARA BORGES; GREGORI, FABIO; SOARES, RODRIGO MARTINS. Evidence of heterozygosity and recombinant alleles in single cysts of Giardia duodenalis. REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA, v. 25, n. 2, p. 9-pg., 2016-04-01. (11/13472-2)

JULIANA MARTINS AGUIAR; SHEILA OLIVEIRA SILVA; VALDIR AZEVEDO DOS SANTOS; SUELI AKEMI TANIWAKI; TRICIA MARIA FERREIRA DE SOUSA OLIVEIRA; HELENA LAGE FERREIRA; LARA BORGES KEID; FÁBIO GREGORI; RODRIGO MARTINS SOARES. Evidência de heterozigose e alelos recombinantes em cistos individualizados de Giardia duodenalis. Revista Brasileira de Parasitologia Veterinária, v. 25, n. 2, p. 187-195, 2016-06-07. (11/13472-2)

AGUIAR, JULIANA MARTINS; DA SILVA, SHEILA OLIVEIRA; DOS SANTOS, VALDIR ANTONIO; RAZERA ROSSA, GISELLE AYRES; TANIWAKI, SUELI AKEMI; GREGORI, FABIO; KEID, LARA BORGES; SOARES, RODRIGO MARTINS. Multilocus amplification of genomic DNA from single cysts of Giardia duodenalis separated using micromanipulation technique. Experimental Parasitology, v. 157, p. 84-87, OCT 2015. (11/13472-2)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

AGUIAR, Juliana Martins. Genotyping of Giardia duodenalis: detection of mixed infection and genetic recombination in samples of human origin. 2015. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) São Paulo.