Analysis of lymphoid and myeloid differentiation potential of B-1 cells in vitro: role of Wnt/beta-catenin signaling pathway

• Grant number: 11/23464-7
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: March 01, 2012
• End date: November 04, 2015
• Field of knowledge: Biological Sciences - Immunology
• Principal Investigator: Ana Flavia Popi
• Grantee: Lika Osugui
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil

Abstract

Hematopoietic Stem Cells are pluripotent cells, which are self-renewing and able to give rise to all lineages of the blood. Although intracellular factors and extrinsic signals play an important role in differentiation of HSC to mature blood cells, the complete mechanism of cellular commitment remains unclear. Wnt signaling pathway is known to regulate many fundamental developmental processes, it is also involved in embryonic cells development and commitment, in addition to be highly conserved during evolution. Recently, studies demonstrated the presence of signal transduction by Wnt pathway in lymphocyte development and HSC self-renewal. Data from our group have showed that B-1 cells express components of Wnt/beta-catenin pathway, like Wnt ligants and receptors. We suggested that Wnt/beta-catenin pathway plays an important role in B-1 self-renewal in vitro, and its inhibition could induce myeloid differentiation in B-1 cells. In order to understand the implications of Wnt pathway in B-1 cells differentiation in vitro, this work aims to investigate the effects of canonical and non-canonical activation of Wnt/beta-catenin pathway in this process, using Wnt ligants, Wnt3a and Wnt5a.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

OSUGUI, LIKA; DE ROO, JOLANDA J.; DE OLIVEIRA, VIVIAN CRISTINA; PIRES SODRE, ANA CLARA; STAAL, FRANK J. T.; POPI, ANA FLAVIA. B-1 cells and B-1 cell precursors prompt different responses to Wnt signaling. PLoS One, v. 13, n. 6, JUN 21 2018. (11/23464-7)