Analysis of glycosylated proteins and metabolites in oral squamous cell carcinomas

• Grant number: 11/23353-0
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: March 01, 2012
• End date: October 31, 2015
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Eloiza Helena Tajara da Silva
• Grantee: Giovana Mussi Polachini
• Host Institution: Faculdade de Medicina de São José do Rio Preto (FAMERP). Secretaria de Desenvolvimento Econômico (São Paulo - Estado). São José do Rio Preto , SP, Brazil
• Associated research grant: 10/51168-0 - Environmental, clinical, histopathological and molecular factors associated with development and prognosis of head and neck squamous cell carcinomas, AP.TEM

Abstract

Oral squamous cell carcinoma (OSCC) is one of the most frequent neoplasias and its main risk factors are alcohol and tobacco exposure. Despite intense research and recent advances in detection and therapeutic strategies, the five-year survival rate remains low in this group of tumors. Genomics and Proteomics have provided powerful tools to study the biology of many cancers including OSCC. While these approaches focus on upstream genetic and protein variations, Metabolomics searches for global metabolic changes that occur in response to pathological, environmental or lifestyle factors. Protein glycosylation is a very common post-translational modification. Most cell surface receptors are glycosylated and there are many evidences that N-glycans modulate their conformation and activity. Aberrant protein glycosylation has been associated with several diseases including cancer. As for Proteome, Metabolome alterations have been related to cancer. The objective of the present study is to search for differentially expressed glycosylated proteins and metabolites in oral squamous cell carcinoma that can be used as biomarkers for prognosis and monitoring of the disease progression. The study will be carried out on oral squamous cell carcinoma cell lines, primary tumor samples and plasma/serum from patients and healthy controls, combining liquid chromatography and mass spectrometry, nuclear magnetic resonance spectroscopy and different validation experiments and functional assays. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

POLACHINI, GIOVANA MUSSI; DE CASTRO, TIALFI BERGAMIN; SMARRA, LUIS FABIANO SOARES; HENRIQUE, TIAGO; DE PAULA, CARLOS HENRIQUE DINIZ; SEVERINO, PATRICIA; LOPEZ, ROSSANA VERONICA MENDOZA; CARVALHO, ANDRE LOPES; DE MATTOS ZERI, ANA CAROLINA; SILVA, ISMAEL DALE COTRIM GUERREIRO; et al. Plasma metabolomics of oral squamous cell carcinomas based on NMR and MS approaches provides biomarker identification and survival prediction. SCIENTIFIC REPORTS, v. 13, n. 1, p. 21-pg., 2023-05-26. (19/07293-0, 21/07087-0, 21/03647-1, 11/23353-0, 10/51168-0)