Development and validation of quantitative NMR methods for drugs, agrochemicals and olive oils analyses

• Grant number: 11/18032-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: March 01, 2012
• End date: February 28, 2014
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Luiz Alberto Colnago
• Grantee: Maiara da Silva Santos
• Host Institution: Embrapa Instrumentação Agropecuária. Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA). Ministério da Agricultura, Pecuária e Abastecimento (Brasil). São Carlos , SP, Brazil
• Associated scholarship(s): 12/11841-3 - Development of new quantitative and qualitative methodologies by NMR for analysis of milk thistle extracts, dietary supplements and phytoconstituents, BE.EP.DR

Abstract

The need for effective and reliable quality control in final products and/or raw materials from pharmaceutical, agrochemical and food industries makes the analyses of its actives and constituents very important to manufacturers as well as to users of these products.For this purpose standard methods such as pharmacopoeia and from governmental agencies are used. Among these methods are the chromatographic and spectroscopic such as UV Visible and infrared. However nuclear magnetic resonance spectroscopy (NMR), which is an important qualitative analytical tool is rarely used in quantitative measurements.Therefore, to increase the use of quantitative NMR (qNMR) it is necessary to validate of NMR methodologies, to be recognized by regulatory agencies, such as ANVISA, IBAMA, INMETRO and MAPA. In this context, the "Resolução RE nº 899 - Guia para Validação de Métodos Analíticos e Bioanalíticos" requirements must be attended, when considered the analysis of drugs and olive oils, and for the agrochemical analyses should be attended the parameters on normative "NBR 14029 - Agrotóxicos e afins: Validação de Métodos Analíticos". While new methods for seed analysis should be compared with reference methods, which are standardized, and to agree with the "Regras para Análise de Sementes".Therefore, the objective of the project is to validate the NMR methodologies for drugs, agrochemical and olive oils, attending the requirements the Brazilian regulatory agencies. (AU)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SANTOS, Maiara da Silva. Development of NMR methods for quality control of pharmaceutical and agricultural. 2014. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Química de São Carlos (IQSC/BT) São Carlos.