Transcriptome analysis of Xylella fastidiosa strains isolated from distinct hosts and search for regulatory RNAs

• Grant number: 11/24091-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: April 01, 2012
• End date: March 31, 2016
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Aline Maria da Silva
• Grantee: Paulo Marques Pierry
• Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Pathogenic bacteria use gene regulation specific mechanisms to rapidly respond to changes in the hostile environment of their hosts. Traditionally, these regulations have been credited to the activities of protein transcription factors that activate and deactivate sets of genes relevant to survival in the host. However, some recent studies suggest that non-coding RNAs (ncRNAs) also play a role in regulating mechanisms of bacterial pathogenesis. Our group recently sequenced the genome of six South American strains of the phytopathogen Xylella fastidiosa, three isolated from citrus (J1a12, U24D and Fb7) and three strains isolated from plum (Pr8x), coffee (3124) and hibiscus (Hib4). The comparative analysis of these X. fastidiosa genomes allowed us to associate genotypic differences to the phenotypes of the distinct isolates. In this project, we propose an extension of these studies by analyzing the complete transcriptome of these strains through RNAseq. The full analysis of the transcriptome will enable the discovery of new genes, particularly ncRNAs, a more precise definition of the structure of genes and operons as well as the detection of non-translated regions, including riboswitches and binding sites of small regulatory RNAs (sRNAs). The expression of some sRNAs candidates identified will be confirmed. We also intend to identify targets of regulation by such ncRNAs. The phenotypic analysis of strains of X. fastidiosa will be further analysed by electron microscopy and adhesion and motility assays in microfluidic chambers. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FEITOSA-JUNIOR, OSCIAS R.; STEFANELLO, ELIEZER; ZAINI, PAULO A.; NASCIMENTO, RAFAEL; PIERRY, PAULO M.; DANDEKAR, ABHAYA M.; LINDOW, STEVEN E.; DA SILVA, ALINE M.. Proteomic and Metabolomic Analyses of Xylella fastidiosa OMV-Enriched Fractions Reveal Association with Virulence Factors and Signaling Molecules of the DSF Family. PHYTOPATHOLOGY, v. 109, n. 8, p. 1344-1353, AUG 2019. (11/24091-0, 08/11703-4)

FEITOSA-JUNIOR, OSEIAS R.; SOUZA, ANA PAULA S.; ZAINI, PAULO A.; BACCARI, CLELIA; IONESCU, MICHAEL; PIERRY, PAULO M.; UCEDA-CAMPOS, GUILLERMO; LABROUSSAA, FABIEN; ALMEIDA, RODRIGO P. P.; LINDOW, STEVEN E.; et al. The XadA Trimeric Autotransporter Adhesins in Xylella fastidiosa Differentially Contribute to Cell Aggregation, Biofilm Formation, Insect Transmission and Virulence to Plants. MOLECULAR PLANT-MICROBE INTERACTIONS, v. 35, n. 9, p. 10-pg., 2022-09-01. (11/09409-3, 11/24091-0, 14/03617-1, 10/16409-7, 08/11703-4)

DE SOUZA, JESSICA BRITO; ALMEIDA-SOUZA, HEBREIA OLIVEIRA; ZAINI, PAULO ADRIANO; ALVES, MONICA NELI; DE SOUZA, ALINE GOMES; PIERRY, PAULO MARQUES; DA SILVA, ALINE MARIA; GOULART, LUIZ RICARDO; DANDEKAR, ABHAYA M.; NASCIMENTO, RAFAEL. Xylella fastidiosasubsp.paucaStrains Fb7 and 9a5c from Citrus Display Differential Behavior, Secretome, and Plant Virulence. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 21, n. 18, SEP 2020. (11/24091-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

PIERRY, Paulo Marques. Comparative transcriptomic of Xylella fastidiosa strains. 2017. Doctoral Thesis - Universidade de São Paulo (USP). Conjunto das Químicas (IQ e FCF) (CQ/DBDCQ) São Paulo.