Study of interfering factors in development and final size of plants female reproductive organ

• Grant number: 11/51844-9
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Effective date (Start): March 01, 2012
• Effective date (End): February 28, 2014
• Field of knowledge: Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
• Principal Investigator: Maria Helena de Souza Goldman
• Grantee: Hebréia Oliveira Almeida Souza
• Host Institution: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

This project intends to study the molecular mechanisms through which the hormone auxin and the proteins SCI1 (Stigma/style Cell-cycle Inhibitor 1) and TOB092H06 influence cell division and expansion, regulating the development and the final size of the female reproductive organ (pistil). Therefore, the objectives of this project are: 1) To study the effects of decreased auxin levels in pistil development: a. To produce N. tabacum transgenic plants with decreased pistil auxin levels, through the expression of the iaaL gene of Agrobacterium tumefaciens, under the control of ST1G1, a stigma-specific promoter; b. To analyze the phenotype of transgenic plants comparing them with the wild-type (SR1), mainly concerning pistil morphology and reproductive parameters; c. Perform the separation by two-dimensional electrophoresis of pistil protein extracts of STIG1::iaaL transgenic and SR1 control plants and analyze the differentially expressed proteins, which will be identified by MALDI-TOF. 2) To characterize the SCI1 protein: a. To express the recombinant SCI1 in E. coli; b. To produce antibody against the recombinant SCI1 protein; c. To compare the phosphorylated residues of SCI1 extracted from leaves and pistils of 35S::GFP-SCI1 transgenic plants. 3) To study interactions between SCI1 and proteins that interfere with cell division: a. To test the interaction by two-hybrid between cyclin L and cyclin-related with the CDKG;2 cyclin-dependent kinase; b. If the proteins interact to test the activity of CDKG;2 on histones and/or other possible substrate of CDK; c. If a and b are positive to test the inhibition of CDKG activity by SCI1. 4) To study the protein encoded by the clone TOB092H06 and its role on pistil development: a. To express the 092H06 recombinant protein in an heterologous system, as E. coli; b. To produce antibodies against the 092H06 recombinant protein; c. To quantify the 092H06 protein in transgenic and control plants. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

STRINI, EDWARD J.; BERTOLINO, LIGIA T.; SAN MARTIN, JUCA A. B.; SOUZA, HEBREIA A. O.; PESSOTTI, FRANCINE; PINOTI, VITOR F.; FERREIRA, PEDRO B.; DE PAOLI, HENRIQUE C.; LUBINI, GREICE; DEL-BEM, LUIZ-EDUARDO; et al. Stigma/Style Cell-Cycle Inhibitor 1, a Regulator of Cell Proliferation, Interacts With a Specific 14-3-3 Protein and Is Degraded During Cell Division. FRONTIERS IN PLANT SCIENCE, v. 13, p. 14-pg., 2022-04-04. (19/24774-1, 16/20486-3, 12/50562-2, 11/51844-9)