| Grant number: | 12/00235-5 |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |
| Start date: | March 01, 2012 |
| End date: | June 30, 2015 |
| Field of knowledge: | Biological Sciences - Genetics - Human and Medical Genetics |
| Principal Investigator: | Paulo Arruda |
| Grantee: | Izabella Agostinho Pena |
| Host Institution: | Centro de Biologia Molecular e Engenharia Genética (CBMEG). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil |
| Associated scholarship(s): | 13/12271-9 - Expression, purification and structural characterization of human lysine-ketoglutarate reductase/Saccharopine dehydrogenase (LKR/SDH), BE.EP.DD |
Abstract Saccharopine pathway of lysine degradation is being studied for many decades in a great variety of organisms and there has been convincing evidences that link this pathway to cellular responses to stress conditions. In plants and mammals, superexpression of Saccharopine pathway genes provided protection against osmotic and oxidative stress. In plants there is a significant increase in expression of the pathway genes in several stress conditions. In mammals, the inducibility of this pathway under stress conditions still has not been tested, and the molecular mechanisms that regulate the expression of the Saccharopine pathway were not explored until now. Cellular stresses like oxidative stress are in focus in many research areas and there are many evidences that correlate stresses as causes of many high-prevalence diseases such as cancer, arteriosclerosis, obesity, Alzheimer's and other neurodegenerative diseases. If Saccharopine pathway is really linked to cellular stress responses, and if their induction mechanisms are deciphered, this knowledge can be very useful not only in basic research and understanding of this pathway physiology but also in therapeutic purposes, to create new stress response-modulating methods. This research project aims to decipher the mechanisms which regulate the induction of Saccharopine pathway and also their relation to stress. Complementarily, this project aims to find the putative transcription factors and/or inducer molecules that act on this way. Bioinformatics and experimental procedures using human cell cultures (HEK293 cells) will be used to this project development. Additionally, the study of this pathway in human cells will complement the studies which are being realized in plants and bacteria, enabling a general concept concerning the saccharopine pathway modulation mechanisms in different biological models. (AU) | |
| News published in Agência FAPESP Newsletter about the scholarship: | |
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