Analysis of melatonina in women with infertility and submitted to program of in vitro fertilization: functional study in granulosa cells

• Grant number: 11/51581-8
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: April 01, 2012
• End date: February 01, 2018
• Field of knowledge: Health Sciences - Medicine - Maternal and Child Health
• Principal Investigator: José Maria Soares Junior
• Grantee: Carla Cristina Maganhin
• Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 14/50457-0 - Melatonin and the control of energy metabolism: basic, clinical and epidemiological research, AP.TEM
• Associated scholarship(s): 16/11131-7 - Analysis of melatonin in women with infertility under program in vitro fertilization: functional study in células da granulosa, BE.EP.PD

Abstract

Melatonin is responsible for carrying the photoperiodic information to the body. This hormone passes this Information on variations in the length of the night during the year (seasonal variations) to the body, allowing it to determine appropriate responses to environmental changes. Therefore, regulates various cellular functions, including the regulation of reproductive function. In addition, the concentration of melatonin is three times higher in follicular fluid than in blood circulation, which may generate a speculation on the melatonin action during ovulation. More recent studies have identified melatonin receptors type I and II in granulosa cells, as well as this hormone acts through cyclic AMP and interacts with the estrogen in rat cell culture. However, little is known about the gene and molecular mechanisms that melatonin influence the function of granulosa cells that may impact the fertility. For this reason, we propose the functional study to assess the molecular pathways and gene action of melatonin on ovarian granulosa cells of women with infertility and submitted to treatment in vitro fertilization program. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MAGANHIN, CARLA C.; BARACAT, MARIA CANDIDA P.; CARVALHO, KATIA C.; SEGANFREDO, ISADORA BRAGA; LUQUETTI, CAMILLA MAGANHIN; SIMOES, RICARDO DOS SANTOS; FERRAZ CARBONEL, ADRIANA APARECIDA; SIMOES, MANUEL DE JESUS; CIPOLLA-NETO, JOSE; BATISTA CASTELLO GIRAO, MANOEL JOAO; et al. Evidence that Melatonin Increases Inhibin Beta-A and Follistatin Gene Expression in Ovaries of Pinealectomized Rats. REPRODUCTIVE SCIENCES, v. 27, n. 7, FEB 2020. (11/51581-8)

MAGANHIN, C. C.; SIMOES, R. S.; BARACAT, M. C. P.; CALIO, M. L.; FUCHS, L. F. P.; SASSO, G. R. S.; GIRAO, J. H. R. C.; BARACAT, E. C.; SOARES JR, J. M.. Immunoexpression of ERK2/p42 MAPK in the ovary of female rats treated with melatonin in the proestrous. GIORNALE ITALIANO DI OSTETRICIA E GINECOLOGIA, v. 36, n. 1, p. 3-pg., 2014-01-01. (11/51581-8, 12/50489-3)

LIMA, GISELE NEGRO; MAGANHIN, CARLA CRISTINA; SIMOES, RICARDO SANTOS; PINHEIRO BARACAT, MARIA CANDIDA; DA SILVA SASSO, GISELA RODRIGUES; PORTUGAL FUCHS, LUIZ FERNANDO; SIMOES, MANUEL DE JESUS; BARACAT, EDMUND CHADA; SOARES JUNIOR, JOSE MARIA. Steroidogenesis-related gene expression in the rat ovary exposed to melatonin supplementation. Clinics, v. 70, n. 2, p. 144-151, 2015. (11/51581-8)