Influence of lycopene on adiponectin levels in adipose tissue of animals subject to hyper caloric diet

• Grant number: 11/19934-8
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: April 01, 2012
• End date: March 31, 2013
• Field of knowledge: Health Sciences - Nutrition - Nutrition Biochemistry
• Principal Investigator: Camila Renata Corrêa
• Grantee: Natália Carla Martins Miranda
• Host Institution: Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

In obesity, the increased oxidative stress levels can be a cause of decreased adiponectin through Akt activation. Given the importance of oxidative stress in pathogenesis of adipose tissue dysfunction, therapeutic strategy using lycopene, a potent antioxidant, may be relevant in an attempt to reduce the ROS overproduction and minimize the consequences of dysfunctional adipocyte. The aim of this study is to analyze the effects of lycopene supplementation on adiponectin levels, via Nox 4 and Akt, in adipose tissue of animals subjected to hyper caloric diet. Male Wistar rats (n=28) will be randomly separated for receiving control diet (C) or hyper caloric diet (H) for 6 weeks. After this period, all animals will be again randomized into four groups: C (n=7), C supplemented with Lycopene (C+L, n=7), H (n=7) or H supplemented with lycopene (H+L, n=7). The lycopene used will be oil-resin mixed with corn oil (10mg/Kg body weight/day); untreated groups (c and H) will receive corn oil at an equivalent volume (~ 0.5 mL/kg/day), both via gavage, for 6 weeks. Food and water will be offered ad libitum, and the consumption will be daily recorded. Body weight will be weekly measured. Blood samples will be collected for serum biochemical and hormonal determinations. Adiponectin, Nox 4, and Akt levels will be measure by their gene expression performed by real time PCR method. Data will be expressed by descriptive measures of position and variability. The comparison between groups will be performed by ANOVA complemented by Tukey test. The adopted significance level will be 5%. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LUVIZOTTO, R. A. M.; NASCIMENTO, A. F.; MIRANDA, N. C. M.; WANG, X-D; FERREIRA, A. L. A.. Lycopene-rich tomato oleoresin modulates plasma adiponectin concentration and mRNA levels of adiponectin, SIRT1, and FoxO1 in adipose tissue of obese rats. HUMAN & EXPERIMENTAL TOXICOLOGY, v. 34, n. 6, p. 612-619, JUN 2015. (11/22786-0, 11/19934-8, 10/19746-4, 10/06100-9)