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Effect of lycopene on oxidative stress, inflammatory process and insulin resistance in adipose tissue of animals with nutritional overload

Grant number: 11/19847-8
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): February 01, 2012
Effective date (End): December 31, 2013
Field of knowledge:Health Sciences - Nutrition - Nutrition Biochemistry
Principal researcher:Camila Renata Corrêa
Grantee:Érika Imaizumi
Home Institution: Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil


One of the triggers of adipose tissue dysfunction, which can lead to insulin resistance, is the oxidative stress. Lycopene is a potent antioxidant that appears to contribute to the attenuation of this process. However, has been discussed whether lycopene therapy can reduces or inhibits adipocyte dysfunction, in nutritional overload condition, reducing the inflammation and insulin resistance. The aim of this study is to analyze the effect of lycopene supplementation on oxidative stress, inflammation process and insulin resistance in adipose tissue of rats subjected to nutritional overload. Male Wistar rats (n=28) will be randomly separated for receiving control diet (C) or hypercaloric diet (H) for 6 weeks. After this period, all animals will be again randomized into four groups: C (n=7), C supplemented with Lycopene (C+L, n=7), H (n=7) or H supplemented with lycopene (H+L, n=7). The lycopene used will be oil-resin mixed with corn oil (10mg/Kg body weight/day); untreated groups (c and H) will receive corn oil at an equivalent volume (~ 0.5 mL/kg/day), both via gavage, for 6 weeks. Food and water will be offered ad libitum, and the consumption will be daily recorded. Body weight will be weekly measured. Blood samples will be collected for serum biochemical, inflammatory and hormonal determinations. The antioxidant status will be measured by TAP and the production of reactive species will be determined indirectly by the presence of the end product of lipid peroxidation, malondialdehyde. Inflammatory cells, in adipose tissue, will be verified by histology. Gene expression (MCP-1, TNF-a and IRS-1) will be performed by real time PCR and protein expression (IRS-1) by Western Blotting method. Data will be expressed by descriptive measures of position and variability. The comparison between groups will be performed by ANOVA complemented by Tukey test. The adopted significance level will be 5%. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MELO LUVIZOTTO, RENATA DE AZEVEDO; NASCIMENTO, ANDRE F.; IMAIZUMI, ERIKA; PIERINE, DAMIANA T.; CONDE, SANDRO J.; CORREA, CAMILA R.; YEUM, KYUNG-JIN; FERREIRA, ANA LUCIA A. Lycopene supplementation modulates plasma concentrations and epididymal adipose tissue mRNA of leptin, resistin and IL-6 in diet-induced obese rats. BRITISH JOURNAL OF NUTRITION, v. 110, n. 10, p. 1803-1809, NOV 28 2013. Web of Science Citations: 27.

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