Effects of microcystins present in the crude extract of cyanobacterium, Microcystis aeruginosa, in the liver and muscle of traírão, Hoplias lacerdae.

• Grant number: 12/00728-1
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: June 01, 2012
• End date: March 31, 2015
• Field of knowledge: Agronomical Sciences - Fishery Resources and Fishery Engineering - Inland Water Fishery Resources
• Principal Investigator: Marisa Narciso Fernandes
• Grantee: Marcelo Gustavo Paulino
• Host Institution: Centro de Ciências Biológicas e da Saúde (CCBS). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil

Abstract

A presence of microcystin into water can be fatal to wildlife and domestic animals and results in various diseases in humans. This project aims to determine the susceptibility of the trairão, Hoplias lacerdae, to microcystin present in the crude extract of M. aeruginosa with emphasis on its effects and mechanism of action in liver and muscle of this species and its bioaccumulation as potential risk for human health. Two experimental protocols will be conducted to test the toxicity of microcystin. In the first protocol, juvenile of H. lacerdae will receive intraperitoneally the crude extract of microcystin and after 6, 12, 24, 48 and 96 hours, the plasma, liver and muscle will be sampled and analyzed to evaluate the response time after acute contamination. In the second protocol, the animals will be chronically contamined, every 96 h, for 32 days and the changes in the liver and muscle will be evaluated as well as the toxin bioaccumulation in these tissues. The toxin in the tissues will be identified by inhibition of protein phosphatases (PP1 and PP2A) and the confirmation and quantification of microcystins in the tissues will be done by HPLC-PDA. Its location will be done using immunohistochemistry. Changes in liver and muscle tissue will be evaluated by biochemical analysis of plasma (activity of alanine aminotransferase, aspartate aminotrasferase, phospholipase A2 and concentration of bilirubin and tumor necrosis factor-±); plasma, liver and muscle (acid and alkaline phosphatase, intermediary metabolites); liver and muscle (biomarkers of oxidative stress). Morphological studies will be performed to determine the degree of liver injury using light microscopy techniques (histopathology, immunostaining of apoptotic cells and cell proliferation) and transmission microscopy.

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PAULINO, MARCELO GUSTAVO; TAVARES, DRIELE; TEREZAN, ANA PAULA; SAKURAGUI, MARISE MARGARETH; PESENTI, EMANUELE; GIANI, ALESSANDRA; CESTARI, MARTA MARGARETH; FERNANDES, JOAO BATISTA; FERNANDES, MARISA NARCISO. Biotransformations, Antioxidant System Responses, and Histopathological Indexes in the Liver of Fish Exposed to Cyanobacterial Extract. Environmental Toxicology and Chemistry, v. 39, n. 5, MAR 2020. (12/00728-1)

PAULINO, M. G.; TAVARES, D.; BIECZYNSKI, F.; PEDRAO, P. G.; SOUZA, N. E. S.; SAKURAGUI, M. M.; LUQUET, C. M.; TEREZAN, A. P.; FERNANDES, J. B.; GIANI, A.; et al. Crude extract of cyanobacteria (Radiocystis fernandoi, strain R28) induces liver impairments in fish. AQUATIC TOXICOLOGY, v. 182, p. 91-101, JAN 2017. (12/00728-1)

TAVARES, DRIELE; PAULINO, MARCELO GUSTAVO; TEREZAN, ANA PAULA; FERNANDES, JOAO BATISTA; GIANI, ALESSANDRA; FERNANDES, MARISA NARCISO. Osmoregulatory disturbance in Neotropical fish exposed to the crude extracts of the cyanobacterium, Radiocystis fernandoi. AQUATIC TOXICOLOGY, v. 216, NOV 2019. (12/00728-1)

PAULINO, MARCELO GUSTAVO; ROSSI, PRISCILA ADRIANA; VENTURINI, FRANCINE PERRI; TAVARES, DRIELE; SAKURAGUI, MARISE MARGARETH; MORAES, GILBERTO; TEREZAN, ANA PAULA; FERNANDES, JOAO BATISTA; GIANI, ALESSANDRA; FERNANDES, MARISA NARCISO. Liver dysfunction and energy storage mobilization in traira, Hoplias malabaricus (Teleostei, Erythrinidae) induced by subchronic exposure to toxic cyanobacterial crude extract. ENVIRONMENTAL TOXICOLOGY, v. 37, n. 11, p. 9-pg., 2022-08-03. (12/00728-1)

SAKURAGUI, MARISE MARGARETH; PAULINO, MARCELO GUSTAVO; DA SILVA E SOUZA, NAIARA ELISABETE; TAVARES, DRIELE; TEREZAN, ANA PAULA; PESENTI, EMANUELE; GIANI, ALESSANDRA; FERNANDES, JOAO BATISTA; CESTARI, MARTA MARGARETE; FERNANDES, MARISA NARCISO. Crude extract of cyanobacterium Radiocystis fernandoi strain R28 induces anemia and oxidative stress in fish erythrocytes. Toxicon, v. 169, p. 18-24, NOV 2019. (12/00728-1)

PAULINO, MARCELO GUSTAVO; ROSSI, PRISCILA ADRIANA; VENTURINI, FRANCINE PERRI; TAVARES, DRIELE; DA SILVA SOUZA, NAIARA ELISABETE; SAKURAGUI, MARISE MARGARETH; MORAES, GILBERTO; TEREZAN, ANA PAULA; FERNANDES, JOAO BATISTA; GIANI, ALESSANDRA; et al. Hepatotoxicity and metabolic effects of cellular extract of cyanobacterium Radiocystis fernandoi containing microcystins RR and YR on neotropical fish (Hoplias malabaricus). Chemosphere, v. 175, p. 431-439, MAY 2017. (12/00728-1)

TAVARES, DRIELE; PAULINO, MARCELO GUSTAVO; TEREZAN, ANA PAULA; FERNANDES, JOAO BATISTA; GIANI, ALESSANDRA; FERNANDES, MARISA NARCISO. Biochemical and morphological biomarkers of the liver damage in the Neotropical fish, Piaractus mesopotamicus, injected with crude extract of cyanobacterium Radiocystis fernandoi. Environmental Science and Pollution Research, v. 25, n. 16, p. 15349-15356, JUN 2018. (12/00728-1)