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IDENTIFICATION Plasmodium spp. IN NEOTROPICAL PRIMATES AND Anopheles IN THE CITIES OF ISLAND OF SÃO LUIS AND THE CITY IMPERATRIZ, STATE MARANHÃO.

Grant number: 12/03961-9
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): May 01, 2012
Effective date (End): April 30, 2015
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
Principal Investigator:Rosangela Zacarias Machado
Grantee:Mayra Araguaia Pereira Figueiredo
Home Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

Brazil has shown about 40% of malaria cases in America and states that make up the Amazon account for 99% of cases in the country. In the state of Maranhão malaria is endemic and has greater impact as the main vector of malaria Anopheles darlingi throughout the state, and Anopheles aquasalis on the coast. Thus, the objective is to identify Plasmodium spp. in neotropical primates and Anopheles in the island municipalities of São Luis and the municipality of Imperatriz, state of Maranhão. For microscopic diagnosis will be used to TBS, which allows differentiation of specific parasites and their developmental stages in the peripheral blood, due to its high concentration in blood, and blood smears, which allows better visualization of the structures of the parasite and the changes characteristics of parasitized erythrocytes. The molecular diagnosis is performed by means of polymerase chain reaction (PCR) enabling the identification of conventional and specific real-time PCR, to quantify the parasite DNA, and the amplification-mediated isothermal circuit (LAMP), due to low parasitemia of samples and that these last two techniques are very sensitive. Also there will be evaluated and the frequency of anti-Plasmodium spp. primates in the wild and in captivity, by indirect immunofluorescence assay (IFA) and enzyme linked immunosorbent assay (ELISA test). To a diagnostic immunoassay field is used, which identifies antigens of P. falciparum and / or antigens of other three species that infect man, P. ovale, P. malariae, P. vivax. It is expected as a result identify the species of Plasmodium exist in reservoirs and vectors in the state of Maranhao, Brazil.

Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PEREIRA FIGUEIREDO, MAYRA ARAGUAIA; DI SANTI, SILVIA MARIA; MANRIQUE, WILSON GOMEZ; ANDRE, MARCOS ROGERIO; MACHADO, ROSANGELA ZACARIAS. Serological and molecular techniques applied for identification of Plasmodium spp. in blood samples from nonhuman primates. Revista Brasileira de Parasitologia Veterinária, v. 27, n. 3, p. 363-376, JUL-SEP 2018. Web of Science Citations: 0.
PEREIRA FIGUEIREDO, MAYRA ARAGUAIA; DI SANTI, SILVIA MARIA; MANRIQUE, WILSON GOMEZ; ANDRE, MARCOS ROGERIO; MACHADO, ROSANGELA ZACARIAS. Identification of Plasmodium spp. in Neotropical primates of Maranhense Amazon in Northeast Brazil. PLoS One, v. 12, n. 8 AUG 10 2017. Web of Science Citations: 5.
PEREIRA FIGUEIREDO, MAYRA ARAGUAIA; DI SANTI, SILVIA MARIA; MANRIQUE, WILSON GOMEZ; GONCALVES, LUIZ RICARDO; ANDRE, MARCOS ROGERIO; MACHADO, ROSANGELA ZACARIAS. Molecular identification of Plasmodium spp. and blood meal sources of anophelines in environmental reserves on Sao LuIs Island, state of Maranhao, Brazil. PARASITES & VECTORS, v. 10, APR 26 2017. Web of Science Citations: 2.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.