Study of the intracellular behavior of M. abscessus subsp. bolettii CRM0019

• Grant number: 12/04913-8
• Support Opportunities: Scholarships in Brazil - Master
• Start date: June 01, 2012
• End date: January 31, 2014
• Field of knowledge: Biological Sciences - Microbiology
• Principal Investigator: Sylvia Luisa Pincherle Cardoso Leão
• Grantee: Giovanni Monteiro Ribeiro
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil

Abstract

The current project proposes a continuation of work in scientific initiation project "Characterization of in vitro development of intracellular Mycobacterium abscessus subsp bolletii CRM0019" (FAPESP, process 2011/02268-5).Mycobacterium abscessus is a rapidly growing mycobacteria (RCM), responsible for infections of the skin and soft tissue and lung disease, mainly in patients with cystic fibrosis. Since 2004, several outbreaks of localized infections caused by members of the group M. abscessus were diagnosed in patients undergoing invasive procedures in Brazil. The study of the behavior of M. intracellular abscessus in different host cells, varying the especies and cell type, may provide a more detailed view on the physiological, composicional, fusional and functional state of mycobacteria-containing phagosomes, helping to understand the biological behavior during bacterial infection. Accordingly, in this work we seek to characterize aspects of the intracellular infection of Mycobacterium abscessus subsp bolletii CRM0019, strain of fast-growing mycobacterium related to an outbreak of infections that involved patients in different states of Brazil between 2004 and 2008, compared with the strain reference ATCC 19 977 (M. abscessus subsp. abscessus).In previous experiments we observed that while the strains are phylogenetically, the rate of internalization of ATCC19977 strain in A549 cells was highest for strain ATCC19977 in relation to isolate CRM0019. Furthermore, the course of infection, bacteria of both strains remained viable and multiply.The aim of this work is to continue the characterization of intracellular strain CRM0019, using as main tools immunofluorescence, flow cytometry and counting of colony forming units, allowing at the end, the establishment of new parameters for study the cell biology of RCMs.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RIBEIRO, GIOVANNI MONTEIRO; MATSUMOTO, CRISTIANNE KAYOKO; REAL, FERNANDO; TEIXEIRA, DANIELA; DUARTE, RAFAEL SILVA; MORTARA, RENATO ARRUDA; LEAO, SYLVIA CARDOSO; CARVALHO-WODARZ, CRISTIANE DE SOUZA. Increased survival and proliferation of the epidemic strain Mycobacterium abscessus subsp massiliense CRM0019 in alveolar epithelial cells. BMC Microbiology, v. 17, SEP 13 2017. (09/14665-9, 12/04913-8, 13/16018-6)