Electrospinning technology for the development of embedded scaffolds with natural oils for skin regeneration

• Grant number: 12/09110-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Effective date (Start): August 01, 2012
• Effective date (End): August 31, 2016
• Field of knowledge: Engineering - Chemical Engineering
• Principal Investigator: Edison Bittencourt
• Grantee: Ana Luíza Garcia Millás Massaguer
• Host Institution: Faculdade de Engenharia Química (FEQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

The present invention relates to nanofibers incorporated with natural oils and a method to evaluate the cell viability and biocompatibility of those nanofibers with essays by adhesion and proliferation of fibroblasts. The science of biomaterials and nanotechnology go together in search of new alternatives and improved physical, chemical and mechanical properties of materials related to the high aspect of the surface area to volume ratio and nanometer dimensions that provide optimum performance with a small amount of material. This study aimed to produce three-dimensional and high porosity nanofibers from a mixture of natural and/or synthetic biodegradable, biocompatible and bioresorbable polymers. Focusing on application as bioactive scaffolds for skin regeneration, the interest is to produce structures that mimic the extracellular matrix. The electrospinning is the technology used, the study of the parameters of the equipment and the conditions of the polymer solution will be the primary challenge of the project. To develop bioactive and functional scaffolds therapeutic amounts of natural oils of Copaifera landesdorffi, Cymbopogon nardus and Carapa guianensis species, will be added to the pre-electrospun polymer solutions.These oils have proven healing, analgesic and antimicrobial properties.The nanofibers embedded with natural oils will be characterized according to diameter, porosity and mechanical properties. First will be made tests of biocompatibility and cell viability in vitro, for adhesion, proliferation, differentiation and gene expression, with human dermal fibroblasts and and will begin testing the material in vivo. The influence of these three oils on the process of electrospinning and the fiber structure has been made by the group. The analysis and characterization of the material will be made by optical microscopy, Scanning Electron Microscopy, Thermal Analysis, Gas Chromatography and Real Time PCR.

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MASSAGUER, Ana Luíza Garcia Millás. In vitro and in vivo properties of PLGA scaffolds incorporated with Copaiba oil-resin prepared by electrospinning. 2016. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Faculdade de Engenharia Química Campinas, SP.