Metabolic engineering of Saccharomyces cerevisiae aimed at improving the energetic yield of sucrose metabolism

• Grant number: 12/05548-1
• Support Opportunities: Scholarships in Brazil - Master
• Start date: July 01, 2012
• End date: February 28, 2014
• Field of knowledge: Engineering - Chemical Engineering - Chemical Process Industries
• Principal Investigator: Andreas Karoly Gombert
• Grantee: Wesley Leoricy Marques
• Host Institution: Escola Politécnica (EP). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated scholarship(s): 12/16630-0 - Metabolic engineering of Saccharomyces cerevisiae aimed at improving the energetic yield of sucrose metabolism, BE.EP.MS

Abstract

The biotechnological industry is a growing field since fossil resources are being attached to ecological and geopolitical constraints. In this scenario, Brazil has a major role due to its large experience in the bioethanol industry and sugarcane use as a cheap feedstock. The aim of this work is to optimize Saccharomyces cerevisiae allowing them to occupy a new niche: the production of economically valuable chemicals that require cellular free energy (ATP) on their biosynthesis. In this context, heterologous protein expression will be done. Therefore, this work will potentially contribute to making certain energy demanding chemicals production economically viable. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MARQUES, WESLEY LEORICY; MANS, ROBERT; MARELLA, EKO ROY; CORDEIRO, ROSA LORIZOLLA; VAN DEN BROEK, MARCEL; DARAN, JEAN-MARC G.; PRONK, JACK T.; GOMBERT, ANDREAS K.; VAN MARIS, ANTONIUS J. A.. Elimination of sucrose transport and hydrolysis in Saccharomyces cerevisiae: a platform strain for engineering sucrose metabolism. FEMS Yeast Research, v. 17, n. 1, JAN 2017. (12/05548-1, 14/07962-5)

MARQUES, WESLEY LEORICY; MANS, ROBERT; HENDERSON, RYAN K.; MARELLA, EKO ROY; TER HORST, JOLANDA; DE HULSTER, ERIK; POOLMAN, BERT; DARAN, JEAN-MARC; PRONK, JACK T.; GOMBERT, ANDREAS K.; et al. Combined engineering of disaccharide transport and phosphorolysis for enhanced ATP yield from sucrose fermentation in Saccharomyces cerevisiae. METABOLIC ENGINEERING, v. 45, p. 121-133, JAN 2018. (12/05548-1, 12/16630-0)

MARQUES, WESLEY LEORICY; RAGHAVENDRAN, VIJAYENDRAN; STAMBUK, BORIS UGARTE; GOMBERT, ANDREAS KAROLY. Sucrose and Saccharomyces cerevisiae: a relationship most sweet. FEMS Yeast Research, v. 16, n. 1, FEB 2016. (12/05548-1)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MARQUES, Wesley Leoricy. Metabolic engineering of Saccharomyces cerevisiae aimed at improving the energetic yield of sucrose metabolism.. 2014. Master's Dissertation - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) São Paulo.