Micropropagation by proliferation of axillary and apical buds of physic nut (Jatropha curcas Linn.) from germplasm grown in ex vitro conditions

• Grant number: 11/17329-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: August 01, 2013
• End date: July 31, 2014
• Field of knowledge: Agronomical Sciences - Forestry Resources and Forestry Engineering - Technology and Use of Forest Products
• Principal Investigator: Isaac Stringueta Machado
• Grantee: Henrique Curi Penna
• Host Institution: Faculdade de Ciências Agronômicas (FCA). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

The use of Jatropha curcas L. for the production of biodiesel is very promising for the agribusiness sector; the potential of the species is diverse, such as adaptation to the marginal regions inapt to other cultures, as well as its active principles medicinal of proven effectiveness. In addition, it also contributes to the conservation of the environment to be renewable biomass, capturer of CO2 from the atmosphere, and all combined with the ability to protect the soil against erosion and leaching of nutrients. However, it is estimated it will take between 2 to 5 years to have the first improved varieties, the micropropagation can speed up conventional methods of vegetative propagation and assist in the development and multiplication of elite genotypes selected in plant-breeding programs. However, the pertinent scientific literature is scarce and there are no research data on seedlings of Jatropha curcas L. produced in vitro. This work aims the development of laboratory protocol for micropropagation of physic nut (Jatropha curcas L.), by multiplication of shoots through axillary and apical buds proliferation; collected from selected promising plants in ex vitro germplasm bank of the DRN / FCA / UNESP-Botucatu / SP. To evaluate the induction of proliferation of shoots, it will be compared the plant growth regulators benzyl amino purine(BAP) and kinetin(KIN) in different concentrations and balances, added to Murashige and Skoog's basal medium nutritive: 0.50 mg.L-1 BAP (T1) 1.00 mg.L-1 BAP (T2), 2.00 mg.L-1 BAP (T3), 3.00 mg.L-1 BAP (T4) and 0.5 mg L-1 KIN (T5), 1.00 mg L-1 KIN (T6), 2.00 mg L-1 KIN (T7), 3.00 mg L-1 KIN (T8), all treatments will be supplemented with the auxin indolyl-butyric acid(AIB) at a concentration of 0.25 mg.L-1. After treatment of disinfection and protection anti-oxidant, axillary and apical buds selected will be inoculated in different treatments, under aseptic conditions of a laminar flow chamber, and then arranged in a growth chamber under controlled environmental conditions. The results may allow the preparation of laboratory protocol and flowchart of the production of seedlings with greater speed, anatomical uniformity of the booth, genetic fidelity to selected matrices, and cleaning of possible phytopathogens endogenous (clonal cleansing); desirable characteristics for the productive sector agribusiness and the possibility of inclusion of small rural producers, for the greater accessibility generated by the increased supply of seedlings.(AU)