Micropropagation of the Brunfelsia uniflora (Pohl) D. Don, an wild Brazilian species in expansion of their use as ornamental plant

• Grant number: 18/02595-5
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: May 01, 2018
• End date: August 31, 2018
• Field of knowledge: Agronomical Sciences - Agronomy - Floriculture, Parks and Gardens
• Principal Investigator: Jean Carlos Cardoso
• Grantee: Ana Victoria Conde da Silva de Matos
• Host Institution: Centro de Ciências Agrárias (CCA). Universidade Federal de São Carlos (UFSCAR). Araras , SP, Brazil

Abstract

The species Brunfelsia uniflora (Pohl) D. Don, common named as manacá, is a native species of occurrence throughout South America. It has ornamental characteristics that can be used in landscaping, besides contributing to the urban afforestation. Thus, with the growing demand of the market for ornamental plants for new species and/or native species, it is necessary to obtain protocols for large scale propagation, due to the inherent difficulties of each species, as is the case of manacá, which produces few seeds and their cuttings rooted in a very limited percentage. In this way, this work will aim to establish a protocol aiming at micropropagation, by obtaining free shoots of contaminations, followed by their multiplication and in vitro rooting as a way to evaluate the viability of the technique aiming at its propagation. The plants obtained will be used later in commercial production to compare the seedlings from micropropagation with those from the cuttings. The culture establishment will be carried out with two culture media, WPM (Wood Plant Medium) and MS ½, reconciled with three doses of Benzyladenine (BA). For the multiplication experiments will be carried out in the absence of regulators, and with the addition of the cytokinins BA and Thidiazuron in the culture medium. Finally, in the rooting phase, the seedlings obtained will be tested with types and concentrations of auxins. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CONDE DA SILVA DE MATOS, ANA VICTORIA; DE OLIVEIRA, BARBARA SAMANTHA; BARBOZA SOUZA DE OLIVEIRA, MARIA EDUARDA; CARDOSO, JEAN CARLOS. AgNO3 improved micropropagation and stimulate in vitro flowering of rose (Rosa x hybrida) cv. Sena. ORNAMENTAL HORTICULTURE-REVISTA BRASILEIRA DE HORTICULTURA ORNAMENTAL, v. 27, n. 1, p. 33-40, JAN-MAR 2021. (19/00243-7, 18/02595-5, 18/07122-8)